Simultaneous determination of twelve quinones from Rubiae radix et Rhizoma before and after carbonization processing by UPLC-MS/MS and their antithrombotic effect on zebrafish.
Antithrombotic effect
Carbonization processing
Simultaneous quantitation
Journal
Journal of pharmaceutical and biomedical analysis
ISSN: 1873-264X
Titre abrégé: J Pharm Biomed Anal
Pays: England
ID NLM: 8309336
Informations de publication
Date de publication:
30 Nov 2020
30 Nov 2020
Historique:
received:
08
05
2020
revised:
27
07
2020
accepted:
08
09
2020
pubmed:
28
9
2020
medline:
22
6
2021
entrez:
27
9
2020
Statut:
ppublish
Résumé
Rubiae Radix et Rhizoma (called "Qiancao", QC), the root and rhizome of Rubia cordifolia L., has been widely used in clinical practice for its excellent performance in removing blood stasis and haemostasis. However, after carbonization processing, significant changes occurred in chemical components of the charcoal of Rubiae Radix et Rhizoma (called "Qiancaotan", QCT), which enhanced the performance in haemostasis and weakened the performance in removing blood stasis in clinic. In order to study the material basis of function variation during processing, a rapid, reliable, accurate and validated UPLC-MS/MS approach was established to determine twelve quinones in QC and QCT simultaneously. Meanwhile, the antithrombotic effect of target components on zebrafish thrombus model induced by phenylhydrazine (PHZ) was investigated. Chromatographic separation was accomplished on an ACQUITY UPLC C
Identifiants
pubmed: 32980794
pii: S0731-7085(20)31524-7
doi: 10.1016/j.jpba.2020.113638
pii:
doi:
Substances chimiques
Drugs, Chinese Herbal
0
Fibrinolytic Agents
0
Quinones
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113638Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare no conflicts of interest.