An isolated retrograde-perfused newborn mouse heart preparation.
Cannulation
Heart
Isolated
Langendorff
Model
Mouse
Newborn
Journal
MethodsX
ISSN: 2215-0161
Titre abrégé: MethodsX
Pays: Netherlands
ID NLM: 101639829
Informations de publication
Date de publication:
2020
2020
Historique:
received:
30
06
2020
accepted:
02
09
2020
entrez:
28
9
2020
pubmed:
29
9
2020
medline:
29
9
2020
Statut:
epublish
Résumé
The Langendorff-perfused model is a powerful tool to study biological responses in the isolated heart in the absence of confounders. The model has been adapted recently to enable study of the isolated mouse heart and the effects of genetic manipulation. Unfortunately, the small size and fragility of the mouse heart pose significant challenges, limiting application of the Langendorff model to the study of adult mice. Cardiac development is a complex and dynamic process that is incompletely understood. Thus, establishing an isolated-perfused heart model in the newborn mouse would be an important and necessary advance. Here we present a method to successfully cannulate and perfuse the isolated newborn murine heart. We describe the basic and fundamental physiological characteristics of the
Identifiants
pubmed: 32983923
doi: 10.1016/j.mex.2020.101058
pii: S2215-0161(20)30278-8
pmc: PMC7492986
doi:
Types de publication
Journal Article
Langues
eng
Pagination
101058Subventions
Organisme : NICHD NIH HHS
ID : R01 HD082251
Pays : United States
Organisme : NINDS NIH HHS
ID : R01 NS112706
Pays : United States
Informations de copyright
© 2020 The Authors.
Déclaration de conflit d'intérêts
None.
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