Citicoline Protects Auditory Hair Cells Against Neomycin-Induced Damage.
aminoglycosides
apoptosis
citicoline
hair cell
reactive oxygen species
Journal
Frontiers in cell and developmental biology
ISSN: 2296-634X
Titre abrégé: Front Cell Dev Biol
Pays: Switzerland
ID NLM: 101630250
Informations de publication
Date de publication:
2020
2020
Historique:
received:
02
04
2020
accepted:
13
07
2020
entrez:
28
9
2020
pubmed:
29
9
2020
medline:
29
9
2020
Statut:
epublish
Résumé
Aminoglycoside-induced hair cell (HC) loss is one of the most important causes of hearing loss. After entering the inner ear, aminoglycosides induce the production of high levels of reactive oxygen species (ROS) that subsequently activate apoptosis in HCs. Citicoline, a nucleoside derivative, plays a therapeutic role in central nervous system injury and in neurodegenerative disease models, including addictive disorders, stroke, head trauma, and cognitive impairment in the elderly, and has been widely used in the clinic as an FDA approved drug. However, its effect on auditory HCs remains unknown. Here, we used HC-like HEI-OC-1 cells and whole organ explant cultured mouse cochleae to explore the effect of citicoline on aminoglycoside-induced HC damage. Consistent with previous reports, both ROS levels and apoptosis were significantly increased in neomycin-induced cochlear HCs and HEI-OC-1 cells compared to undamaged controls. Interestingly, we found that co-treatment with citicoline significantly protected against neomycin-induced HC loss in both HEI-OC-1 cells and whole organ explant cultured cochleae. Furthermore, we demonstrated that citicoline could significantly reduce neomycin-induced mitochondrial dysfunction and inhibit neomycin-induced ROS accumulation and subsequent apoptosis. Thus, we conclude that citicoline can protect against neomycin-induced HC loss by inhibiting ROS aggregation and thus preventing apoptosis in HCs, and this suggests that citicoline might serve as a potential therapeutic drug in the clinic to protect HCs.
Identifiants
pubmed: 32984303
doi: 10.3389/fcell.2020.00712
pmc: PMC7487320
doi:
Types de publication
Journal Article
Langues
eng
Pagination
712Informations de copyright
Copyright © 2020 Zhong, Fu, Li, Chen, Wang, Gao, Zhang, Cheng, Zhang, Li, Zhang, Qian, Shu, Chai and Gao.
Références
J Physiol. 2005 Sep 1;567(Pt 2):359-60
pubmed: 16037084
N Engl J Med. 1993 Oct 7;329(15):1092-102
pubmed: 8371732
Clin Interv Aging. 2013;8:131-7
pubmed: 23403474
J Clin Invest. 2016 Sep 1;126(9):3556-66
pubmed: 27500493
Audiol Neurootol. 2002 May-Jun;7(3):171-4
pubmed: 12053140
J Neurochem. 2013 Sep;126(6):819-26
pubmed: 23600725
Mol Ther. 2004 Feb;9(2):173-81
pubmed: 14759801
Front Med. 2016 Jun;10(2):143-51
pubmed: 27189205
Integr Biol (Camb). 2011 Sep;3(9):879-86
pubmed: 21799993
Int J Mol Sci. 2014 Apr 14;15(4):6286-97
pubmed: 24736780
J Assoc Res Otolaryngol. 2013 Oct;14(5):645-59
pubmed: 23821348
Mol Neurobiol. 2017 Mar;54(2):1326-1339
pubmed: 26843109
Nat Protoc. 2017 Jan;12(1):183-194
pubmed: 28005069
PLoS One. 2013 Nov 06;8(11):e79817
pubmed: 24223197
Curr Opin Otolaryngol Head Neck Surg. 2003 Oct;11(5):328-33
pubmed: 14502062
Sci Rep. 2017 Jan 23;7:41094
pubmed: 28112219
Sci Rep. 2016 Jul 13;6:29621
pubmed: 27405449
Methods Find Exp Clin Pharmacol. 2006 Sep;28 Suppl B:1-56
pubmed: 17171187
Biochimie. 2002 Feb-Mar;84(2-3):187-93
pubmed: 12022949
Biochim Biophys Acta. 2010 Jun-Jul;1797(6-7):678-97
pubmed: 20096261
J Neurosci. 2010 Mar 17;30(11):3933-46
pubmed: 20237264
Int J Dev Neurosci. 2000 Apr-Jun;18(2-3):259-70
pubmed: 10715580
Audiol Neurootol. 2003 Jul-Aug;8(4):177-89
pubmed: 12811000
Hear Res. 1996 Sep 1;98(1-2):116-24
pubmed: 8880186
Oncotarget. 2016 Oct 11;7(41):66754-66768
pubmed: 27564256
Neuroreport. 2002 Nov 15;13(16):2109-11
pubmed: 12438935
Autophagy. 2017;13(11):1884-1904
pubmed: 28968134
Nature. 2001 Mar 29;410(6828):549-54
pubmed: 11279485
Apoptosis. 2014 Dec;19(12):1712-26
pubmed: 25342393
Exp Eye Res. 2006 Nov;83(5):1128-34
pubmed: 16876158
Cell Death Differ. 2009 Oct;16(10):1303-14
pubmed: 19662025
Front Med. 2016 Sep;10(3):237-49
pubmed: 27527363
Am J Drug Alcohol Abuse. 2014 Jul;40(4):262-8
pubmed: 24950234
J Neurochem. 2002 Jan;80(1):12-23
pubmed: 11796739
Dev Cell. 2011 Jul 19;21(1):92-101
pubmed: 21763611
Cell Death Dis. 2016 Mar 10;7:e2136
pubmed: 26962686
Prog Brain Res. 2008;173:541-54
pubmed: 18929133
Mech Ageing Dev. 2020 Jan;185:111191
pubmed: 31765645
J Neurosci. 2002 Feb 15;22(4):1218-27
pubmed: 11850449
Neuron. 2014 Feb 5;81(3):471-83
pubmed: 24507186
Brain Res. 2002 Dec 6;957(1):84-90
pubmed: 12443983
Hear Res. 2009 May;251(1-2):70-82
pubmed: 19286452
Cell Death Differ. 2012 Feb;19(2):267-73
pubmed: 21720385
Mol Neurobiol. 2015 Feb;51(1):252-67
pubmed: 24781382
Cell Death Dis. 2015 Jan 22;6:e1605
pubmed: 25611380
Hear Res. 2007 Apr;226(1-2):157-67
pubmed: 17113254
Neurobiol Aging. 2019 Jul;79:30-42
pubmed: 31026620
Yonsei Med J. 2008 Oct 31;49(5):689-97
pubmed: 18972587
Mol Aspects Med. 2010 Jun;31(3):227-85
pubmed: 20346371
Science. 2004 Feb 13;303(5660):1010-4
pubmed: 14963330
Curr Pharm Des. 2006;12(18):2249-70
pubmed: 16787253
Oxid Med Cell Longev. 2017;2017:2825703
pubmed: 29163753
Nature. 2015 Aug 20;524(7565):309-314
pubmed: 26266977
Neuroscience. 2009 Jun 16;161(1):214-26
pubmed: 19318119
Oncotarget. 2016 Oct 11;7(41):66647-66659
pubmed: 27556499
Sci Rep. 2013 Oct 21;3:2996
pubmed: 24141260
J Biol Chem. 2002 Aug 23;277(34):30574-80
pubmed: 12058035
J Comp Neurol. 2004 Jul 12;475(1):1-18
pubmed: 15176081
Mol Neurobiol. 2015 Aug;52(1):196-205
pubmed: 25132091
Front Cell Neurosci. 2014 Sep 15;8:248
pubmed: 25278835
Cell Death Differ. 2015 Mar;22(3):377-88
pubmed: 25257172
Redox Biol. 2020 Jan;28:101364
pubmed: 31731101
Cell Death Dis. 2015 Apr 02;6:e1710
pubmed: 25837486
Rev Neurol Dis. 2008 Fall;5(4):167-77
pubmed: 19122569
Hear Res. 2000 Apr;142(1-2):34-40
pubmed: 10748326
Brain Res. 2006 Apr 7;1081(1):203-10
pubmed: 16696125