A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid.


Journal

Molecular microbiology
ISSN: 1365-2958
Titre abrégé: Mol Microbiol
Pays: England
ID NLM: 8712028

Informations de publication

Date de publication:
02 2021
Historique:
received: 09 05 2020
revised: 16 09 2020
accepted: 17 09 2020
pubmed: 29 9 2020
medline: 31 8 2021
entrez: 28 9 2020
Statut: ppublish

Résumé

The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpG

Identifiants

pubmed: 32985020
doi: 10.1111/mmi.14614
pmc: PMC7984374
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

255-271

Informations de copyright

© 2020 The Authors. Molecular Microbiology published by John Wiley & Sons Ltd.

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Auteurs

Shady Mansour Kamal (SM)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.
Department of Microbiology and Immunology, Faculty of Pharmaceutical Sciences & Pharmaceutical Industries, Future University in Egypt, Cairo, Egypt.

Annika Cimdins-Ahne (A)

Institute of Hygiene, University of Münster, Münster, Germany.

Changhan Lee (C)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Fengyang Li (F)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Alberto J Martín-Rodríguez (AJ)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

Zaira Seferbekova (Z)

Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia.
Faculty of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Moscow, Russia.

Robert Afasizhev (R)

Kharkevich Institute for Information Transmission Problems, RAS, Moscow, Russia.

Haleluya Tesfaye Wami (HT)

Institute of Hygiene, University of Münster, Münster, Germany.

Panagiotis Katikaridis (P)

Center for Molecular Biology, University of Heidelberg (ZMBH), German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.

Lena Meins (L)

Center for Molecular Biology, University of Heidelberg (ZMBH), German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.

Heinrich Lünsdorf (H)

Helmholtz Centre for Infection Research, Braunschweig, Germany.

Ulrich Dobrindt (U)

Institute of Hygiene, University of Münster, Münster, Germany.

Axel Mogk (A)

Center for Molecular Biology, University of Heidelberg (ZMBH), German Cancer Research Center (DKFZ), DKFZ-ZMBH Alliance, Heidelberg, Germany.

Ute Römling (U)

Department of Microbiology, Tumor and Cell Biology, Karolinska Institutet, Stockholm, Sweden.

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