Prognostic Significance of TYRO3 Receptor Tyrosine Kinase Expression in Gastric Cancer.
Aged
Biomarkers, Tumor
/ genetics
Carcinogenesis
/ genetics
Cell Line, Tumor
Cell Movement
/ genetics
Cell Proliferation
/ genetics
Disease Progression
Female
Gene Expression Regulation, Neoplastic
Humans
Middle Aged
Neoplasm Invasiveness
/ genetics
Prognosis
Proto-Oncogene Proteins c-akt
/ genetics
Receptor Protein-Tyrosine Kinases
/ genetics
Stomach Neoplasms
/ diagnosis
TAM
TYRO3
gastric cancer
prognostic factor
Journal
Anticancer research
ISSN: 1791-7530
Titre abrégé: Anticancer Res
Pays: Greece
ID NLM: 8102988
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
17
07
2020
revised:
08
08
2020
accepted:
11
08
2020
entrez:
29
9
2020
pubmed:
30
9
2020
medline:
8
10
2020
Statut:
ppublish
Résumé
Despite improved treatment for gastric cancer (GC), the prognosis of advanced disease remains poor. Further investigation of the oncogenic sequence for GC is needed. The expression of TYRO3 protein tyrosine kinase in five GC cell lines was confirmed using western blotting. TYRO3 knockdown in GC cells, and bromodeoxyuridine and Transwell assays were used to examine the functions of TYRO3 in tumor proliferation and invasion. Finally, TYRO3 expression in 138 patients who underwent curative gastric resection for advanced GC (Union for International Cancer Control stage II/III) was tested by immunohistochemistry, and the association between prognosis and TYRO3 expression was analyzed. TYRO3 was detected at various levels in all the tested GC cell lines. Deleting TYRO3 significantly suppressed proliferation and invasion. Immunohistochemistry revealed TYRO3 expression was an independent prognostic factor for overall survival in patients with GC. TYRO3 appears to mediate tumor progression and predict prognosis of patients with GC.
Sections du résumé
BACKGROUND
BACKGROUND
Despite improved treatment for gastric cancer (GC), the prognosis of advanced disease remains poor. Further investigation of the oncogenic sequence for GC is needed.
MATERIALS AND METHODS
METHODS
The expression of TYRO3 protein tyrosine kinase in five GC cell lines was confirmed using western blotting. TYRO3 knockdown in GC cells, and bromodeoxyuridine and Transwell assays were used to examine the functions of TYRO3 in tumor proliferation and invasion. Finally, TYRO3 expression in 138 patients who underwent curative gastric resection for advanced GC (Union for International Cancer Control stage II/III) was tested by immunohistochemistry, and the association between prognosis and TYRO3 expression was analyzed.
RESULTS
RESULTS
TYRO3 was detected at various levels in all the tested GC cell lines. Deleting TYRO3 significantly suppressed proliferation and invasion. Immunohistochemistry revealed TYRO3 expression was an independent prognostic factor for overall survival in patients with GC.
CONCLUSION
CONCLUSIONS
TYRO3 appears to mediate tumor progression and predict prognosis of patients with GC.
Identifiants
pubmed: 32988883
pii: 40/10/5593
doi: 10.21873/anticanres.14572
doi:
Substances chimiques
Biomarkers, Tumor
0
Receptor Protein-Tyrosine Kinases
EC 2.7.10.1
TYRO3 protein, human
EC 2.7.10.1
Proto-Oncogene Proteins c-akt
EC 2.7.11.1
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
5593-5600Informations de copyright
Copyright© 2020, International Institute of Anticancer Research (Dr. George J. Delinasios), All rights reserved.