Structures of B. subtilis Maturation RNases Captured on 50S Ribosome with Pre-rRNAs.
B. subtilis
M5
Mini-III
RNA
RNase
cryo-electron microscopy
maturation
ribosome
structure
Journal
Molecular cell
ISSN: 1097-4164
Titre abrégé: Mol Cell
Pays: United States
ID NLM: 9802571
Informations de publication
Date de publication:
15 10 2020
15 10 2020
Historique:
received:
12
03
2020
revised:
16
07
2020
accepted:
04
09
2020
pubmed:
30
9
2020
medline:
30
10
2020
entrez:
29
9
2020
Statut:
ppublish
Résumé
The pathways for ribosomal RNA (rRNA) maturation diverge greatly among the domains of life. In the Gram-positive model bacterium, Bacillus subtilis, the final maturation steps of the two large ribosomal subunit (50S) rRNAs, 23S and 5S pre-rRNAs, are catalyzed by the double-strand specific ribonucleases (RNases) Mini-RNase III and RNase M5, respectively. Here we present a protocol that allowed us to solve the 3.0 and 3.1 Å resolution cryoelectron microscopy structures of these RNases poised to cleave their pre-rRNA substrates within the B. subtilis 50S particle. These data provide the first structural insights into rRNA maturation in bacteria by revealing how these RNases recognize and process double-stranded pre-rRNA. Our structures further uncover how specific ribosomal proteins act as chaperones to correctly fold the pre-rRNA substrates and, for Mini-III, anchor the RNase to the ribosome. These r-proteins thereby serve a quality-control function in the process from accurate ribosome assembly to rRNA processing.
Identifiants
pubmed: 32991829
pii: S1097-2765(20)30616-X
doi: 10.1016/j.molcel.2020.09.008
pmc: PMC7610893
mid: EMS124734
pii:
doi:
Substances chimiques
Bacterial Proteins
0
RNA Precursors
0
Ribonucleases
EC 3.1.-
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
227-236.e5Subventions
Organisme : Wellcome Trust
ID : 200873
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 200873/Z/16/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 206171/Z/17/Z
Pays : United Kingdom
Organisme : Wellcome Trust
ID : 202905/Z/16/Z
Pays : United Kingdom
Informations de copyright
Copyright © 2020 Elsevier Inc. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Interests The authors declare no competing interests.
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