PKM2 Determines Myofiber Hypertrophy In Vitro and Increases in Response to Resistance Exercise in Human Skeletal Muscle.


Journal

International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791

Informations de publication

Date de publication:
25 Sep 2020
Historique:
received: 09 08 2020
revised: 21 09 2020
accepted: 23 09 2020
entrez: 30 9 2020
pubmed: 1 10 2020
medline: 26 2 2021
Statut: epublish

Résumé

Nearly 100 years ago, Otto Warburg investigated the metabolism of growing tissues and discovered that tumors reprogram their metabolism. It is poorly understood whether and how hypertrophying muscle, another growing tissue, reprograms its metabolism too. Here, we studied pyruvate kinase muscle (PKM), which can be spliced into two isoforms (PKM1, PKM2). This is of interest, because PKM2 redirects glycolytic flux towards biosynthetic pathways, which might contribute to muscle hypertrophy too. We first investigated whether resistance exercise changes PKM isoform expression in growing human skeletal muscle and found that PKM2 abundance increases after six weeks of resistance training, whereas PKM1 decreases. Second, we determined that

Identifiants

pubmed: 32992783
pii: ijms21197062
doi: 10.3390/ijms21197062
pmc: PMC7583908
pii:
doi:

Substances chimiques

Carrier Proteins 0
Membrane Proteins 0
Thyroid Hormones 0

Types de publication

Journal Article

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : Fedeal Institute of Sports Sciences (BISP)
ID : BISP AZ 070106/16-17 / BISP IIA1-070112/13-14

Références

Genes Dev. 1998 Aug 15;12(16):2499-509
pubmed: 9716403
Nat Commun. 2020 Jan 24;11(1):470
pubmed: 31980607
Acta Physiol (Oxf). 2008 Apr;192(4):541-9
pubmed: 17970825
Am J Physiol Cell Physiol. 2009 Oct;297(4):C1019-27
pubmed: 19657059
J Physiol. 2005 Oct 1;568(Pt 1):283-90
pubmed: 16051622
Biomed Environ Sci. 2009 Oct;22(5):359-68
pubmed: 20163059
Front Physiol. 2014 Jan 31;5:30
pubmed: 24550841
Biomed Imaging Interv J. 2009 Apr;5(2):e7
pubmed: 21611031
Am J Clin Nutr. 2006 Sep;84(3):475-82
pubmed: 16960159
Mol Cancer. 2018 Feb 19;17(1):35
pubmed: 29455645
J Gen Physiol. 1927 Mar 7;8(6):519-30
pubmed: 19872213
Biochem Pharmacol. 2010 Apr 15;79(8):1118-24
pubmed: 20005212
J Appl Physiol (1985). 2010 Jun;108(6):1825
pubmed: 20519722
Arch Neurol. 1970 Oct;23(4):369-79
pubmed: 4248905
Cancer Metab. 2018 May 31;6:6
pubmed: 29854399
Cell Cycle. 2013 Oct 1;12(19):3154-8
pubmed: 24013426
J Am Geriatr Soc. 2002 May;50(5):889-96
pubmed: 12028177
Am J Physiol. 1983 Mar;244(3):C276-87
pubmed: 6829750
Cell. 2011 Mar 4;144(5):646-74
pubmed: 21376230
Cell Biosci. 2019 Jun 26;9:52
pubmed: 31391918
Clin Nucl Med. 2015 Dec;40(12):975-6
pubmed: 26284769
Cell Cycle. 2015;14(10):1559-67
pubmed: 25790097
Biochim Biophys Acta Rev Cancer. 2019 Apr;1871(2):331-341
pubmed: 30826427
Nat Commun. 2016 Oct 10;7:13041
pubmed: 27721378
Curr Opin Clin Nutr Metab Care. 2012 May;15(3):240-5
pubmed: 22466926
Science. 1956 Aug 10;124(3215):270-2
pubmed: 13351640
Muscle Nerve. 2000 Jul;23(7):1095-104
pubmed: 10883005
Am J Physiol Cell Physiol. 2020 Aug 1;319(2):C300-C312
pubmed: 32520607
Eur J Appl Physiol. 2012 Jul;112(7):2393-402
pubmed: 22038147
Front Physiol. 2014 Feb 03;5:32
pubmed: 24567722
J Physiol. 2017 Aug 1;595(15):5209-5226
pubmed: 28542873
Clin Sci (Lond). 1991 Aug;81(2):249-56
pubmed: 1716189
J Proteome Res. 2016 Feb 5;15(2):499-509
pubmed: 26732810
Cell. 2017 Mar 9;168(6):960-976
pubmed: 28283069
Nature. 2010 Jan 21;463(7279):364-8
pubmed: 20010808
Mol Cell. 2019 May 2;74(3):609-621.e6
pubmed: 30922843
J Cell Mol Med. 2019 Oct;23(10):6846-6858
pubmed: 31343107
Biochem J. 2016 Dec 15;473(24):4527-4550
pubmed: 27941030
Eur J Appl Physiol. 2010 Nov;110(4):665-94
pubmed: 20602111
Med Sci Sports Exerc. 2006 Nov;38(11):1950-7
pubmed: 17095929
Oncotarget. 2016 Jul 26;7(30):48155-48167
pubmed: 27340866
Proc Natl Acad Sci U S A. 2013 Aug 13;110(33):13570-5
pubmed: 23901116
Cold Spring Harb Symp Quant Biol. 2011;76:325-34
pubmed: 22262476
Biochem J. 1999 Apr 15;339 ( Pt 2):443-51
pubmed: 10191278
F1000 Biol Rep. 2012;4:2
pubmed: 22242042
Mol Cell. 2015 Jan 8;57(1):95-107
pubmed: 25482511
Am J Physiol Endocrinol Metab. 2015 Jul 1;309(1):E72-83
pubmed: 25968575
J Biol Chem. 1986 Oct 15;261(29):13807-12
pubmed: 3020052
J Strength Cond Res. 2010 Oct;24(10):2857-72
pubmed: 20847704
Pflugers Arch. 2015 Jun;467(6):1343-56
pubmed: 25070178
Physiol Rev. 2011 Oct;91(4):1447-531
pubmed: 22013216
Med Sci Sports Exerc. 1982;14(1):101-2
pubmed: 7070249
J Biol Chem. 2015 Oct 30;290(44):26445-56
pubmed: 26370082
FEBS J. 2013 Sep;280(17):4004-13
pubmed: 23402377
J Appl Physiol (1985). 2019 Jan 1;126(1):30-43
pubmed: 30335577
Am J Physiol. 1999 Jan;276(1):C120-7
pubmed: 9886927
Biochimie. 1977;59(1):65-71
pubmed: 870081
J Appl Physiol (1985). 2019 Aug 1;127(2):581-590
pubmed: 30676865
Biochim Biophys Acta Mol Cell Res. 2017 Jan;1864(1):62-75
pubmed: 27756573
EMBO Rep. 2016 Dec;17(12):1721-1730
pubmed: 27856534
Int J Sports Physiol Perform. 2010 Dec;5(4):497-508
pubmed: 21266734
Sci Adv. 2016 May 27;2(5):e1600200
pubmed: 27386546
Genes Dev. 2016 May 1;30(9):1020-33
pubmed: 27125672

Auteurs

Sander A J Verbrugge (SAJ)

Department for Sport and Health Sciences, Technical University of Munich, Georg-Brauchle-Ring 60/62, 80992 München/Munich, Germany.

Sebastian Gehlert (S)

Department for the Biosciences of Sports, Institute of Sports Science, University of Hildesheim, Universitätsplatz 1, 31141 Hildesheim, Germany.
Department for Molecular and Cellular Sports Medicine, German Sport University Cologne, 50933 Cologne, Germany.

Lian E M Stadhouders (LEM)

Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Daniel Jacko (D)

Department for Molecular and Cellular Sports Medicine, German Sport University Cologne, 50933 Cologne, Germany.

Thorben Aussieker (T)

Department for Molecular and Cellular Sports Medicine, German Sport University Cologne, 50933 Cologne, Germany.

Gerard M J de Wit (G)

Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Ilse S P Vogel (ISP)

Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Carla Offringa (C)

Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Martin Schönfelder (M)

Department for Sport and Health Sciences, Technical University of Munich, Georg-Brauchle-Ring 60/62, 80992 München/Munich, Germany.

Richard T Jaspers (RT)

Laboratory for Myology, Department of Human Movement Sciences, Faculty of Behavioural and Movement Sciences, Vrije Universiteit Amsterdam, Amsterdam Movement Sciences, De Boelelaan 1108, 1081 HZ Amsterdam, The Netherlands.

Henning Wackerhage (H)

Department for Sport and Health Sciences, Technical University of Munich, Georg-Brauchle-Ring 60/62, 80992 München/Munich, Germany.

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Classifications MeSH