Proteome of larval metamorphosis induced by epinephrine in the Fujian oyster Crassostrea angulata.
Crassostrea angulata
Epinephrine
Metamorphosis
Proteomic
Settlement
Journal
BMC genomics
ISSN: 1471-2164
Titre abrégé: BMC Genomics
Pays: England
ID NLM: 100965258
Informations de publication
Date de publication:
29 Sep 2020
29 Sep 2020
Historique:
received:
03
02
2020
accepted:
10
09
2020
entrez:
30
9
2020
pubmed:
1
10
2020
medline:
15
4
2021
Statut:
epublish
Résumé
The Fujian oyster Crassostrea angulata is an economically important species that has typical settlement and metamorphosis stages. The development of the oyster involves complex morphological and physiological changes, the molecular mechanisms of which are as yet unclear. In this study, changes in proteins were investigated during larval settlement and metamorphosis of Crassostrea angulata using epinephrine induction. Protein abundance and identity were characterized using label-free quantitative proteomics, tandem mass spectrometry (MS/ MS), and Mascot methods. The results showed that more than 50% (764 out of 1471) of the quantified proteins were characterized as differentially expressed. Notably, more than two-thirds of the differentially expressed proteins were down-regulated in epinephrine-induced larvae. The results showed that "metabolic process" was closely related to the development of settlement and metamorphosis; 5 × 10 The study provides insight into proteins that function in energy metabolism, immune responses, settlement and metamorphosis, and shell formation in C. angulata. The results contribute valuable information for further research on larval settlement and metamorphosis.
Sections du résumé
BACKGROUND
BACKGROUND
The Fujian oyster Crassostrea angulata is an economically important species that has typical settlement and metamorphosis stages. The development of the oyster involves complex morphological and physiological changes, the molecular mechanisms of which are as yet unclear.
RESULTS
RESULTS
In this study, changes in proteins were investigated during larval settlement and metamorphosis of Crassostrea angulata using epinephrine induction. Protein abundance and identity were characterized using label-free quantitative proteomics, tandem mass spectrometry (MS/ MS), and Mascot methods. The results showed that more than 50% (764 out of 1471) of the quantified proteins were characterized as differentially expressed. Notably, more than two-thirds of the differentially expressed proteins were down-regulated in epinephrine-induced larvae. The results showed that "metabolic process" was closely related to the development of settlement and metamorphosis; 5 × 10
CONCLUSION
CONCLUSIONS
The study provides insight into proteins that function in energy metabolism, immune responses, settlement and metamorphosis, and shell formation in C. angulata. The results contribute valuable information for further research on larval settlement and metamorphosis.
Identifiants
pubmed: 32993483
doi: 10.1186/s12864-020-07066-z
pii: 10.1186/s12864-020-07066-z
pmc: PMC7525975
doi:
Substances chimiques
Calmodulin
0
Calreticulin
0
Proteome
0
Mitogen-Activated Protein Kinases
EC 2.7.11.24
Epinephrine
YKH834O4BH
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
675Subventions
Organisme : the Chinese Ministry of Science and Technology through the National Key Research and Development Program of China
ID : 2018YFD0901400
Organisme : The Earmarked Fund for Modern Agro-industry Technology Research System
ID : CARS-49
Organisme : National Marine Economic Development Demonstration Project in Xiamen
ID : 16CZB023SF12
Organisme : the Key Project of Science and Technology Research of Henan Provincial Department of Education
ID : 20A240002
Organisme : National Natural Science Foundation of China
ID : 31672651
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