Collection of immature Dermacentor reticulatus (Fabricius, 1794) ticks from vegetation and detection of Rickettsia raoultii in them.
Dermacentor reticulatuslarvae
Rickettsia spp.
flagging
nymphs
Journal
Ticks and tick-borne diseases
ISSN: 1877-9603
Titre abrégé: Ticks Tick Borne Dis
Pays: Netherlands
ID NLM: 101522599
Informations de publication
Date de publication:
11 2020
11 2020
Historique:
received:
17
05
2020
revised:
04
08
2020
accepted:
19
08
2020
entrez:
30
9
2020
pubmed:
1
10
2020
medline:
30
6
2021
Statut:
ppublish
Résumé
It is commonly assumed that Dermacentor reticulatus immature life stages are nidicolous and therefore cannot be collected from vegetation. However, in June and July of 2018 and 2019, a total of 47 questing D. reticulatus larvae and two nymphs were collected by the flagging method in two different sites close to the city of Leipzig, Germany. To confirm their role in the transmission of tick-borne pathogens, 45 larvae (pooled by 2 in 21 pools and 1 pool with three individuals) and one nymph were tested either by conventional or real-time PCR for the presence of Bartonella spp., Neoehrlichia mikurensis, Rickettsia spp., and Babesia spp. All samples tested negative for Bartonella spp., N. mikurensis, and Babesia spp.; while the minimal infection rate of larvae for Rickettsia spp. was 42%, and the one tested nymph was also positive. Sequencing partial ompB genes revealed the presence of Rickettsia raoultii in larvae and nymph. Further research needs to be done to determine under which circumstances immature D. reticulatus ticks are found outside the burrows of their hosts and can be collected from vegetation.
Identifiants
pubmed: 32993950
pii: S1877-959X(20)30412-X
doi: 10.1016/j.ttbdis.2020.101543
pii:
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
101543Informations de copyright
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