Eye lymphatic defects induced by bone morphogenetic protein 9 deficiency have no functional consequences on intraocular pressure.
Animals
Anterior Chamber
/ physiology
Aqueous Humor
/ metabolism
Glaucoma
/ metabolism
Growth Differentiation Factor 2
/ metabolism
Intraocular Pressure
/ physiology
Lymphangiogenesis
/ physiology
Lymphatic Vessels
/ metabolism
Male
Membrane Transport Proteins
/ metabolism
Mice
Mice, Inbred C57BL
Sclera
/ physiology
Tonometry, Ocular
/ methods
Trabecular Meshwork
/ physiology
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
29 09 2020
29 09 2020
Historique:
received:
05
03
2020
accepted:
18
08
2020
entrez:
30
9
2020
pubmed:
1
10
2020
medline:
26
1
2021
Statut:
epublish
Résumé
Aqueous humor drainage is essential for the regulation of intraocular pressure (IOP), a major risk factor for glaucoma. The Schlemm's canal and the non-conventional uveoscleral pathway are known to drain aqueous humor from the eye anterior chamber. It has recently been reported that lymphatic vessels are involved in this process, and that the Schlemm's canal responds to some lymphatic regulators. We have previously shown a critical role for bone morphogenetic protein 9 (BMP9) in lymphatic vessel maturation and valve formation, with repercussions in drainage efficiency. Here, we imaged eye lymphatic vessels and analyzed the consequences of Bmp9 (Gdf2) gene invalidation. A network of lymphatic vessel hyaluronan receptor 1 (LYVE-1)-positive lymphatic vessels was observed in the corneolimbus and the conjunctiva. In contrast, LYVE-1-positive cells present in the ciliary bodies were belonging to the macrophage lineage. Although enlarged conjunctival lymphatic trunks and a reduced valve number were observed in Bmp9-KO mice, there were no morphological differences in the Schlemm's canal compared to wild type animals. Moreover, there were no functional consequences on IOP in both basal control conditions and after laser-induced ocular hypertonia. Thus, the BMP9-activated signaling pathway does not constitute a wise target for new glaucoma therapeutic strategies.
Identifiants
pubmed: 32994463
doi: 10.1038/s41598-020-71877-z
pii: 10.1038/s41598-020-71877-z
pmc: PMC7524742
doi:
Substances chimiques
Growth Differentiation Factor 2
0
Membrane Transport Proteins
0
Xlkd1 protein, mouse
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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