Improved production of Humira antibody in the genetically engineered Escherichia coli SHuffle, by co-expression of human PDI-GPx7 fusions.
Disulfide bond
E. coli
GPx7
Humira production
PDI
Redox engineering
SHuffle
Journal
Applied microbiology and biotechnology
ISSN: 1432-0614
Titre abrégé: Appl Microbiol Biotechnol
Pays: Germany
ID NLM: 8406612
Informations de publication
Date de publication:
Nov 2020
Nov 2020
Historique:
received:
03
07
2020
accepted:
18
09
2020
revised:
11
09
2020
pubmed:
1
10
2020
medline:
8
5
2021
entrez:
30
9
2020
Statut:
ppublish
Résumé
Microbial production of antibodies offers the promise of cheap, fast, and efficient production of antibodies at an industrial scale. Limiting this capacity in prokaryotes is the absence of the post-translational machinery, present in dedicated antibody producing eukaryotic cell lines, such as B cells. There has been few and limited success in producing full-length, correctly folded, and assembled IgG in the cytoplasm of prokaryotic cell lines. One such success was achieved by utilizing the genetically engineered Escherichia coli strain SHuffle with an oxidative cytoplasm. Due to the genetic disruption of reductive pathways, SHuffle cells are under constant oxidative stress, including increased levels of hydrogen peroxide (H
Identifiants
pubmed: 32997203
doi: 10.1007/s00253-020-10920-5
pii: 10.1007/s00253-020-10920-5
pmc: PMC7595990
doi:
Substances chimiques
Escherichia coli Proteins
0
Hydrogen Peroxide
BBX060AN9V
Peroxidases
EC 1.11.1.-
GPX7 protein, human
EC 1.11.1.9
Glutathione Peroxidase
EC 1.11.1.9
Protein Disulfide-Isomerases
EC 5.3.4.1
Adalimumab
FYS6T7F842
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
9693-9706Références
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