Inhibitory Potential of Polyclonal Camel Antibodies against New Delhi Metallo-β-lactamase-1 (NDM-1).
NDM-1
antibiotic resistance
camel antibodies
metallo-β-lactamases
potential inhibitors
Journal
Molecules (Basel, Switzerland)
ISSN: 1420-3049
Titre abrégé: Molecules
Pays: Switzerland
ID NLM: 100964009
Informations de publication
Date de publication:
28 Sep 2020
28 Sep 2020
Historique:
received:
30
07
2020
revised:
08
09
2020
accepted:
09
09
2020
entrez:
1
10
2020
pubmed:
2
10
2020
medline:
20
3
2021
Statut:
epublish
Résumé
New Delhi Metallo-β-lactamase-1 (NDM-1) is the most prevalent type of metallo-β-lactamase, able to hydrolyze almost all antibiotics of the β-lactam group, leading to multidrug-resistant bacteria. To date, there are no clinically relevant inhibitors to fight NDM-1. The use of dromedary polyclonal antibody inhibitors against NDM-1 represents a promising new class of molecules with inhibitory activity. In the current study, immunoreactivities of dromedary Immunoglobulin G (IgG) isotypes containing heavy-chain and conventional antibodies were tested after successful immunization of dromedary using increasing amounts of the recombinant NDM-1 enzyme. Inhibition kinetic assays, performed using a spectrophotometric method with nitrocefin as a reporter substrate, demonstrated that IgG1, IgG2, and IgG3 were able to inhibit not only the hydrolytic activity of NDM-1 but also Verona integron-encoded metallo-β-lactamase (VIM-1) (subclass B1) and L1 metallo-β-lactamase (L1) (subclass B3) with inhibitory concentration (IC
Identifiants
pubmed: 32998307
pii: molecules25194453
doi: 10.3390/molecules25194453
pmc: PMC7584030
pii:
doi:
Substances chimiques
Antibodies
0
Immune Sera
0
Immunoglobulin G
0
beta-lactamase L1
EC 3.5.2.-
beta-Lactamases
EC 3.5.2.6
beta-lactamase NDM-1
EC 3.5.2.6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
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