Journal

Science translational medicine
ISSN: 1946-6242
Titre abrégé: Sci Transl Med
Pays: United States
ID NLM: 101505086

Informations de publication

Date de publication:
30 09 2020
Historique:
received: 31 10 2019
accepted: 06 05 2020
entrez: 1 10 2020
pubmed: 2 10 2020
medline: 24 6 2021
Statut: ppublish

Résumé

Postinfectious hydrocephalus (PIH), which often follows neonatal sepsis, is the most common cause of pediatric hydrocephalus worldwide, yet the microbial pathogens underlying this disease remain to be elucidated. Characterization of the microbial agents causing PIH would enable a shift from surgical palliation of cerebrospinal fluid (CSF) accumulation to prevention of the disease. Here, we examined blood and CSF samples collected from 100 consecutive infant cases of PIH and control cases comprising infants with non-postinfectious hydrocephalus in Uganda. Genomic sequencing of samples was undertaken to test for bacterial, fungal, and parasitic DNA; DNA and RNA sequencing was used to identify viruses; and bacterial culture recovery was used to identify potential causative organisms. We found that infection with the bacterium

Identifiants

pubmed: 32998967
pii: 12/563/eaba0565
doi: 10.1126/scitranslmed.aba0565
pmc: PMC7774825
mid: NIHMS1609375
pii:
doi:

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural

Langues

eng

Sous-ensembles de citation

IM

Subventions

Organisme : NIAID NIH HHS
ID : R01 AI145057
Pays : United States
Organisme : Intramural NIH HHS
ID : ZIA LM000073
Pays : United States
Organisme : Intramural NIH HHS
ID : Z99 LM999999
Pays : United States
Organisme : NCATS NIH HHS
ID : KL2 TR002015
Pays : United States
Organisme : Medical Research Council
ID : MR/M017990/1
Pays : United Kingdom
Organisme : NICHD NIH HHS
ID : DP1 HD086071
Pays : United States
Organisme : NCI NIH HHS
ID : R35 CA220523
Pays : United States
Organisme : Intramural NIH HHS
ID : Z01 LM000073
Pays : United States

Informations de copyright

Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works.

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Auteurs

Joseph N Paulson (JN)

Department of Biostatistics, Product Development, Genentech Inc., South San Francisco, CA 94080, USA.

Brent L Williams (BL)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Department of Epidemiology, Columbia University Mailman School of Public Health, New York, NY 10032, USA.

Christine Hehnly (C)

Institute for Personalized Medicine, Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Nischay Mishra (N)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Department of Epidemiology, Columbia University Mailman School of Public Health, New York, NY 10032, USA.

Shamim A Sinnar (SA)

Center for Neural Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Department of Medicine, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Lijun Zhang (L)

Institute for Personalized Medicine, Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Paddy Ssentongo (P)

Center for Neural Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA 16802, USA.
Department of Public Health Sciences, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Edith Mbabazi-Kabachelor (E)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Dona S S Wijetunge (DSS)

Department of Pathology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Benjamin von Bredow (B)

Department of Pathology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Ronnie Mulondo (R)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Julius Kiwanuka (J)

Department of Pediatrics, Mbarara University of Science and Technology, P.O. Box 1410 Mbarara, Uganda.

Francis Bajunirwe (F)

Department of Epidemiology, Mbarara University of Science and Technology, P.O. Box 1410, Mbarara, Uganda.

Joel Bazira (J)

Department of Microbiology, Mbarara University of Science and Technology, P.O. Box 1410 Mbarara, Uganda.

Lisa M Bebell (LM)

Division of Infectious Disease, Massachusetts Genereal Hospital, Harvard Medical School, 55 Fruit St, GRJ-504, Boston, MA 02114, USA.

Kathy Burgoine (K)

Neonatal Unit, Department of Paediatrics and Child Health, Mbale Regional Referral Hospital, Plot 29-33 Pallisa Road, P.O. Box 1966, Mbale, Uganda.
Mbale Clinical Research Institute, Mbale Regional Referral Hospital, Plot 29-33 Pallisa Road, P.O. Box 1966 Mbale, Uganda.
University of Liverpool, Liverpool, L69 3BX, UK.

Mara Couto-Rodriguez (M)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Biotia, 100 6th avenue, New York, NY 10013, USA.

Jessica E Ericson (JE)

Division of Pediatric Infectious Disease, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Tim Erickson (T)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Matthew Ferrari (M)

Center for Infectious Disease Dynamics, Pennsylvania State University, University Park, PA 16802, USA.
Department of Biology, Pennsylvania State University, University Park, PA 16802, USA.
Department of Statistics, Pennsylvania State University, University Park, PA 16802, USA.

Melissa Gladstone (M)

Institute for Translational Medicine, University of Liverpool, Liverpool, L12 2AP, UK.

Cheng Guo (C)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.

Murali Haran (M)

Department of Statistics, Pennsylvania State University, University Park, PA 16802, USA.

Mady Hornig (M)

Department of Epidemiology, Columbia University Mailman School of Public Health, New York, NY 10032, USA.

Albert M Isaacs (AM)

Department of Neuroscience, Washington University School of Medicine, St. Louis, MO 63130, USA.

Brian Nsubuga Kaaya (BN)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Sheila M Kangere (SM)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Abhaya V Kulkarni (AV)

Division of Neurosurgery, Hospital for Sick Children, University of Toronto, Toronto, Ontario, M5G 1X8, Canada.

Elias Kumbakumba (E)

Department of Pediatrics, Mbarara University of Science and Technology, P.O. Box 1410 Mbarara, Uganda.

Xiaoxiao Li (X)

Institute for Translational Medicine, University of Liverpool, Liverpool, L12 2AP, UK.

David D Limbrick (DD)

Department of Neurological Surgery, Washington University School of Medicine, St. Louis, MO 63130, USA.

Joshua Magombe (J)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Sarah U Morton (SU)

Division of Newborn Medicine, Boston Children's Hospital and Department of Pediatrics, Harvard Medical School, Boston MA 02115, USA.

John Mugamba (J)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

James Ng (J)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.

Peter Olupot-Olupot (P)

Mbale Clinical Research Institute, Mbale Regional Referral Hospital, Plot 29-33 Pallisa Road, P.O. Box 1966 Mbale, Uganda.
Busitema University, Mbale Campus, Plot 29-33 Pallisa Road, P.O. Box 1966, Mbale, Uganda.

Justin Onen (J)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Mallory R Peterson (MR)

Center for Neural Engineering, Pennsylvania State University, University Park, PA 16802, USA.
Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA 16802, USA.

Farrah Roy (F)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.

Kathryn Sheldon (K)

Institute for Personalized Medicine, Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Reid Townsend (R)

Department of Medicine, Washington University School of Medicine , St. Louis, MO 63130, USA.

Andrew D Weeks (AD)

Sanyu Research Unit, Liverpool Women's Hospital, University of Liverpool, Liverpool L8 7SS, UK.

Andrew J Whalen (AJ)

Department of Mechanical Engineering, Pennsylvania State University, University Park, PA 16802, USA.

John Quackenbush (J)

Department of Biostatistics, Harvard T.H. Chan School of Public Health, Boston, MA 02115, USA.

Peter Ssenyonga (P)

CURE Children's Hospital of Uganda, Plot 97-105, Bugwere Road, P.O. Box 903 Mbale, Uganda.

Michael Y Galperin (MY)

National Center for Biotechnology Information, National Library of Medicine, National Institutes of Health, Bethesda, MD 20894, USA.

Mathieu Almeida (M)

Université Paris-Saclay, INRAE, MGP, Jouy-en-Josas, 78350, France.

Hannah Atkins (H)

Department of Comparative Medicine, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Benjamin C Warf (BC)

Department of Neurosurgery, Boston Children's Hospital, Harvard Medical School, Boston, MA 02115, USA.

W Ian Lipkin (WI)

Center for Infection and Immunity, Mailman School of Public Health, Columbia University, New York, NY 10032, USA.
Department of Epidemiology, Columbia University Mailman School of Public Health, New York, NY 10032, USA.

James R Broach (JR)

Institute for Personalized Medicine, Department of Biochemistry and Molecular Biology, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.

Steven J Schiff (SJ)

Center for Neural Engineering, Pennsylvania State University, University Park, PA 16802, USA. steven.j.schiff@gmail.com.
Department of Engineering Science and Mechanics, Pennsylvania State University, University Park, PA 16802, USA.
Center for Infectious Disease Dynamics, Pennsylvania State University, University Park, PA 16802, USA.
Department of Neurosurgery, Pennsylvania State University College of Medicine, Hershey, PA 17033, USA.
Department of Physics, Pennsylvania State University, University Park, PA 16802, USA.

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