UBA2 activates Wnt/β-catenin signaling pathway during protection of R28 retinal precursor cells from hypoxia by extracellular vesicles derived from placental mesenchymal stem cells.
Exosome
Optic nerve injury
Retinal precursor cells
UBA2
Wnt/β-catenin
hPMSCs
Journal
Stem cell research & therapy
ISSN: 1757-6512
Titre abrégé: Stem Cell Res Ther
Pays: England
ID NLM: 101527581
Informations de publication
Date de publication:
02 10 2020
02 10 2020
Historique:
received:
05
06
2020
accepted:
18
09
2020
entrez:
3
10
2020
pubmed:
4
10
2020
medline:
22
6
2021
Statut:
epublish
Résumé
Stem cell transplantation has been proposed as an alternative treatment for intractable optic nerve disorders characterized by irrecoverable loss of cells. Mesenchymal stem cells, with varying tissue regeneration and recovery capabilities, are being considered for potential cell therapies. To overcome the limitations of cell therapy, we isolated exosomes from human placenta-derived mesenchymal stem cells (hPMSCs) and investigated their therapeutic effects in R28 cells (retinal precursor cells) exposed to CoCl After 9 h of exposure to CoCl We observed that exosome could significantly recover proliferation damaged by CoCl This study reported that hypoxic damaged expression of regeneration markers in R28 cells was significantly recovered by hPMSC exosomes. We could also demonstrate that UBA2 played a key role in activating the Wnt/β-catenin signaling pathway during protection of hypoxic damaged R28 cells, induced by hPMSC exosomes.
Sections du résumé
BACKGROUND
Stem cell transplantation has been proposed as an alternative treatment for intractable optic nerve disorders characterized by irrecoverable loss of cells. Mesenchymal stem cells, with varying tissue regeneration and recovery capabilities, are being considered for potential cell therapies. To overcome the limitations of cell therapy, we isolated exosomes from human placenta-derived mesenchymal stem cells (hPMSCs) and investigated their therapeutic effects in R28 cells (retinal precursor cells) exposed to CoCl
METHOD
After 9 h of exposure to CoCl
RESULT
We observed that exosome could significantly recover proliferation damaged by CoCl
CONCLUSION
This study reported that hypoxic damaged expression of regeneration markers in R28 cells was significantly recovered by hPMSC exosomes. We could also demonstrate that UBA2 played a key role in activating the Wnt/β-catenin signaling pathway during protection of hypoxic damaged R28 cells, induced by hPMSC exosomes.
Identifiants
pubmed: 33008487
doi: 10.1186/s13287-020-01943-w
pii: 10.1186/s13287-020-01943-w
pmc: PMC7532108
doi:
Substances chimiques
UBA2 protein, human
0
beta Catenin
0
Ubiquitin-Activating Enzymes
EC 6.2.1.45
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
428Références
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