Temporal airway microbiome changes related to ventilator-associated pneumonia in children.
Journal
The European respiratory journal
ISSN: 1399-3003
Titre abrégé: Eur Respir J
Pays: England
ID NLM: 8803460
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
18
05
2020
accepted:
02
09
2020
pubmed:
4
10
2020
medline:
3
7
2021
entrez:
3
10
2020
Statut:
epublish
Résumé
We sought to determine whether temporal changes in the lower airway microbiome are associated with ventilator-associated pneumonia (VAP) in children.Using a multicentre prospective study of children aged 31 days to 18 years requiring mechanical ventilation support for >72 h, daily tracheal aspirates were collected and analysed by sequencing of the 16S rRNA gene. VAP was assessed using 2008 Centers for Disease Control and Prevention paediatric criteria. The association between microbial factors and VAP was evaluated using joint longitudinal time-to-event modelling, matched case-control comparisons and unsupervised clustering.Out of 366 eligible subjects, 66 (15%) developed VAP at a median of 5 (interquartile range 3-5) days post intubation. At intubation, there was no difference in total bacterial load (TBL), but Shannon diversity and the relative abundance of
Identifiants
pubmed: 33008935
pii: 13993003.01829-2020
doi: 10.1183/13993003.01829-2020
pmc: PMC7979474
mid: NIHMS1659900
pii:
doi:
Substances chimiques
RNA, Ribosomal, 16S
0
Types de publication
Journal Article
Multicenter Study
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NICHD NIH HHS
ID : UG1 HD049983
Pays : United States
Organisme : NICHD NIH HHS
ID : U10 HD049983
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD063108
Pays : United States
Organisme : NICHD NIH HHS
ID : RL1 HD107773
Pays : United States
Organisme : NHLBI NIH HHS
ID : K23 HL138461
Pays : United States
Organisme : NICHD NIH HHS
ID : U01 HD049934
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD049981
Pays : United States
Organisme : NICHD NIH HHS
ID : K23 HD096018
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD050096
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD083170
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD083166
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL124103
Pays : United States
Organisme : NICHD NIH HHS
ID : UG1 HD083171
Pays : United States
Investigateurs
Kathleen L Meert
(KL)
Sabrina M Heidemann
(SM)
Robert A Berg
(RA)
Athena F Zuppa
(AF)
Murray M Pollack
(MM)
Michael Bell
(M)
David L Wessel
(DL)
John T Berger
(JT)
Randall Burd
(R)
Peter M Mourani
(PM)
Todd C Carpenter
(TC)
Mark W Hall
(MW)
Andrew R Yates
(AR)
Anil Sapru
(A)
Patrick McQuillen
(P)
Joseph A Carcillo
(JA)
Ericka L Fink
(EL)
J Michael Dean
(JM)
Richard Holubkov
(R)
Katherine Sward
(K)
Ron W Reeder
(RW)
John VanBuren
(J)
Daniel A Notterman
(DA)
Informations de copyright
Copyright ©ERS 2021.
Déclaration de conflit d'intérêts
Conflict of interest: P.M. Mourani reports grants from NIH NHLBI and NIH NICHD, during the conduct of the study. Conflict of interest: M.K. Sontag reports grants from NIH NHLBI, during the conduct of the study. Conflict of interest: K.M. Williamson has nothing to disclose. Conflict of interest: J.K. Harris has nothing to disclose. Conflict of interest: R. Reeder has nothing to disclose. Conflict of interest: C. Locandro has nothing to disclose. Conflict of interest: T.C. Carpenter reports grants from NIH NHLBI and NIH NICHD, during the conduct of the study. Conflict of interest: A.B. Maddux reports a grant from Parker B. Francis Foundation (Fellowship Award) and NIH/NICHD K23HD096018, outside the submitted work. Conflict of interest: K. Ziegler reports grants from NIH NHLBI during the conduct of the study. Conflict of interest: E.A.F. Simões reports grants from NIH NHLBI, during the conduct of the study. Conflict of interest: C.M. Osborne has nothing to disclose. Conflict of interest: L. Ambroggio has nothing to disclose. Conflict of interest: M.K. Leroue has nothing to disclose. Conflict of interest: C.E. Robertson has nothing to disclose. Conflict of interest: C. Langelier has nothing to disclose. Conflict of interest: J.L. DeRisi reports grants from NIH NHLBI, during the conduct of the study. Conflict of interest: J. Kamm has nothing to disclose. Conflict of interest: M.W. Hall reports grants from NIH NICHD, during the conduct of the study. Conflict of interest: A.F. Zuppa has nothing to disclose. Conflict of interest: J. Carcillo has nothing to disclose. Conflict of interest: K. Meert reports grants from NIH, during the conduct of the study. Conflict of interest: A. Sapru reports grants from NIH NICHD, during the conduct of the study. Conflict of interest: M.M. Pollack reports grants from NIH, during the conduct of the study. Conflict of interest: P. McQuillen reports grants from NIH NICHD, during the conduct of the study. Conflict of interest: D.A. Notterman has nothing to disclose. Conflict of interest: J.M. Dean reports grants from NIH, during the conduct of the study. Conflict of interest: B.D. Wagner reports grants from NIH NHLBI, during the conduct of the study.
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