Mild atopic dermatitis lacks systemic inflammation and shows reduced nonlesional skin abnormalities.
Atopic dermatitis
OLINK
cytokines
immune tolerance
limited
mild
proteomics
qRT-PCR
severity
systemic inflammation
Journal
The Journal of allergy and clinical immunology
ISSN: 1097-6825
Titre abrégé: J Allergy Clin Immunol
Pays: United States
ID NLM: 1275002
Informations de publication
Date de publication:
04 2021
04 2021
Historique:
received:
20
03
2020
revised:
17
07
2020
accepted:
06
08
2020
pubmed:
5
10
2020
medline:
21
9
2021
entrez:
4
10
2020
Statut:
ppublish
Résumé
Molecular studies in atopic dermatitis (AD) are largely restricted to patients with moderate-to-severe disease. Our aim was to evaluate skin and blood abnormalities in mild, moderate, and severe AD. Skin and blood samples were obtained from 61 patients with AD (20 with mild or limited disease, 17 with moderate disease, and 24 with severe disease) and 20 healthy subjects. Immune and barrier markers were measured in lesional, nonlesional, and healthy skin by quantitative real-time PCR and immunohistochemistry, and in blood by using the OLINK proteomic assay. Cellular markers of epidermal hyperplasia and T-cell/dendritic cell infiltration were increased in AD tissues of all patients in all severity groups versus in those of controls, whereas downstream T Mild and limited AD show high levels of T
Sections du résumé
BACKGROUND
Molecular studies in atopic dermatitis (AD) are largely restricted to patients with moderate-to-severe disease.
OBJECTIVE
Our aim was to evaluate skin and blood abnormalities in mild, moderate, and severe AD.
METHODS
Skin and blood samples were obtained from 61 patients with AD (20 with mild or limited disease, 17 with moderate disease, and 24 with severe disease) and 20 healthy subjects. Immune and barrier markers were measured in lesional, nonlesional, and healthy skin by quantitative real-time PCR and immunohistochemistry, and in blood by using the OLINK proteomic assay.
RESULTS
Cellular markers of epidermal hyperplasia and T-cell/dendritic cell infiltration were increased in AD tissues of all patients in all severity groups versus in those of controls, whereas downstream T
CONCLUSION
Mild and limited AD show high levels of T
Identifiants
pubmed: 33011244
pii: S0091-6749(20)31334-8
doi: 10.1016/j.jaci.2020.08.041
pii:
doi:
Substances chimiques
Cytokines
0
Proteome
0
RNA, Messenger
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
1369-1380Informations de copyright
Copyright © 2020 American Academy of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.