UNMIX-ME: spectral and lifetime fluorescence unmixing via deep learning.
Journal
Biomedical optics express
ISSN: 2156-7085
Titre abrégé: Biomed Opt Express
Pays: United States
ID NLM: 101540630
Informations de publication
Date de publication:
01 Jul 2020
01 Jul 2020
Historique:
received:
04
03
2020
revised:
30
04
2020
accepted:
30
04
2020
entrez:
5
10
2020
pubmed:
6
10
2020
medline:
6
10
2020
Statut:
epublish
Résumé
Hyperspectral fluorescence lifetime imaging allows for the simultaneous acquisition of spectrally resolved temporal fluorescence emission decays. In turn, the acquired rich multidimensional data set enables simultaneous imaging of multiple fluorescent species for a comprehensive molecular assessment of biotissues. However, to enable quantitative imaging, inherent spectral overlap between the considered fluorescent probes and potential bleed-through must be considered. Such a task is performed via either spectral or lifetime unmixing, typically independently. Herein, we present "UNMIX-ME" (unmix multiple emissions), a deep learning-based fluorescence unmixing routine, capable of quantitative fluorophore unmixing by simultaneously using both spectral and temporal signatures. UNMIX-ME was trained and validated using an
Identifiants
pubmed: 33014571
doi: 10.1364/BOE.391992
pii: 391992
pmc: PMC7510912
doi:
Types de publication
Journal Article
Langues
eng
Pagination
3857-3874Subventions
Organisme : NCI NIH HHS
ID : R01 CA207725
Pays : United States
Organisme : NCI NIH HHS
ID : R01 CA237267
Pays : United States
Organisme : NIBIB NIH HHS
ID : R01 EB019443
Pays : United States
Informations de copyright
© 2020 Optical Society of America under the terms of the OSA Open Access Publishing Agreement.
Déclaration de conflit d'intérêts
The authors declare no conflicts of interest.
Références
Curr Mol Imaging. 2014;3(2):144-161
pubmed: 26023359
Methods Mol Biol. 2014;1075:129-48
pubmed: 24052349
J Histochem Cytochem. 2000 May;48(5):653-62
pubmed: 10769049
Sci Rep. 2020 Apr 16;10(1):6504
pubmed: 32300110
Nat Methods. 2016 Mar;13(3):257-62
pubmed: 26808668
Nat Photonics. 2017;11:411-414
pubmed: 29242714
J Control Release. 2018 Sep 28;286:451-459
pubmed: 30036545
Light Sci Appl. 2019 Mar 6;8:26
pubmed: 30854198
Biophys J. 2008 Jul;95(1):378-89
pubmed: 18359789
J Phys Chem B. 2009 May 14;113(19):6583-99
pubmed: 19331333
Biophys J. 2005 Oct;89(4):2736-49
pubmed: 16040744
PLoS One. 2013 Nov 21;8(11):e80269
pubmed: 24278268
J Biophotonics. 2019 Mar;12(3):e201800185
pubmed: 30421551
EJNMMI Res. 2018 Apr 4;8(1):26
pubmed: 29619584
Proc Natl Acad Sci U S A. 2019 Nov 26;116(48):24019-24030
pubmed: 31719196