Proinflammatory intervertebral disc cell and organ culture models induced by tumor necrosis factor alpha.
3R
cytokines
inflammation
intervertebral disc
regeneration
spine
Journal
JOR spine
ISSN: 2572-1143
Titre abrégé: JOR Spine
Pays: United States
ID NLM: 101722350
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
received:
31
01
2020
revised:
30
05
2020
accepted:
01
06
2020
entrez:
5
10
2020
pubmed:
6
10
2020
medline:
6
10
2020
Statut:
epublish
Résumé
Inflammation plays an important role in the pathogenesis of intervertebral disc (IVD) degeneration. The proinflammatory cytokine tumor necrosis factor alpha (TNF-α) has shown markedly higher expression in degenerated human disc tissue compared with healthy controls. Anti-inflammatory treatment targeting TNF-α has shown to alleviate discogenic pain in patients with low back pain. Therefore, in vitro and ex vivo inflammatory models utilizing TNF-α provide relevant experimental conditions for drug development in disc degeneration research. The current method article addressed several specific questions related to the model establishment. (a) The effects of bovine and human recombinant TNF-α on bovine nucleus pulposus (NP) cells were compared. (b) The required dose for an inflammatory IVD organ culture model with intradiscal TNF-α injection was studied. (c) The effect of TNF-α blocking at different stages of inflammation was evaluated. Outcomes revealed that bovine and human recombinant TNF-α induced equivalent inflammatory effects in bovine NP cells. A bovine whole IVD inflammatory model was established by intradiscal injection of 100 ng TNF-α/ cm
Identifiants
pubmed: 33015577
doi: 10.1002/jsp2.1104
pii: JSP21104
pmc: PMC7524256
doi:
Types de publication
Journal Article
Langues
eng
Pagination
e1104Informations de copyright
© 2020 The Authors. JOR Spine published by Wiley Periodicals LLC. on behalf of Orthopaedic Research Society.
Déclaration de conflit d'intérêts
The authors have no conflict of interest.
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