Cross-Sectional Evaluation of Humoral Responses against SARS-CoV-2 Spike.

COVID-19 IgG IgM RBD SARS-CoV-2 Spike glycoproteins coronavirus cross-reactivity, IgA neutralization

Journal

Cell reports. Medicine
ISSN: 2666-3791
Titre abrégé: Cell Rep Med
Pays: United States
ID NLM: 101766894

Informations de publication

Date de publication:
20 10 2020
Historique:
received: 12 06 2020
revised: 20 08 2020
accepted: 21 09 2020
pubmed: 6 10 2020
medline: 6 10 2020
entrez: 5 10 2020
Statut: ppublish

Résumé

SARS-CoV-2 is responsible for the coronavirus disease 2019 (COVID-19) pandemic, infecting millions of people and causing hundreds of thousands of deaths. The Spike glycoproteins of SARS-CoV-2 mediate viral entry and are the main targets for neutralizing antibodies. Understanding the antibody response directed against SARS-CoV-2 is crucial for the development of vaccine, therapeutic, and public health interventions. Here, we perform a cross-sectional study on 106 SARS-CoV-2-infected individuals to evaluate humoral responses against SARS-CoV-2 Spike. Most infected individuals elicit anti-Spike antibodies within 2 weeks of the onset of symptoms. The levels of receptor binding domain (RBD)-specific immunoglobulin G (IgG) persist over time, and the levels of anti-RBD IgM decrease after symptom resolution. Although most individuals develop neutralizing antibodies within 2 weeks of infection, the level of neutralizing activity is significantly decreased over time. Our results highlight the importance of studying the persistence of neutralizing activity upon natural SARS-CoV-2 infection.

Identifiants

pubmed: 33015650
doi: 10.1016/j.xcrm.2020.100126
pii: S2666-3791(20)30168-3
pmc: PMC7524645
doi:

Types de publication

Journal Article Research Support, Non-U.S. Gov't Research Support, N.I.H., Extramural

Langues

eng

Pagination

100126

Subventions

Organisme : NIAID NIH HHS
ID : HHSN272201400008C
Pays : United States
Organisme : NIAID NIH HHS
ID : R01 AI122953
Pays : United States

Commentaires et corrections

Type : UpdateOf

Informations de copyright

© 2020 The Author(s).

Déclaration de conflit d'intérêts

The authors declare no competing interests.

Références

Nat Med. 2020 Jul;26(7):1033-1036
pubmed: 32398876
Nat Med. 2020 Aug;26(8):1200-1204
pubmed: 32555424
J Mol Biol. 2020 May 1;432(10):3309-3325
pubmed: 32320687
Curr Protoc Microbiol. 2020 Jun;57(1):e100
pubmed: 32302069
Proc Natl Acad Sci U S A. 2005 May 31;102(22):7988-93
pubmed: 15897467
Science. 2020 May 8;368(6491):630-633
pubmed: 32245784
Nature. 2020 May;581(7807):221-224
pubmed: 32225175
mBio. 2020 Oct 16;11(5):
pubmed: 33067385
Nat Commun. 2020 May 27;11(1):2688
pubmed: 32461612
J Infect Dis. 2020 Jun 29;222(2):183-188
pubmed: 32358956
J Infect. 2020 Sep;81(3):e31-e32
pubmed: 32622905
Cell. 2020 Apr 16;181(2):271-280.e8
pubmed: 32142651
N Engl J Med. 2020 Sep 10;383(11):1085-1087
pubmed: 32706954
Emerg Microbes Infect. 2020 Dec;9(1):382-385
pubmed: 32065055
Science. 2020 Mar 13;367(6483):1260-1263
pubmed: 32075877
Nat Microbiol. 2019 May;4(5):734-747
pubmed: 30886356
Nature. 2020 Aug;584(7819):120-124
pubmed: 32454512
Cell. 2020 Apr 16;181(2):281-292.e6
pubmed: 32155444
Sci Immunol. 2020 May 13;5(47):
pubmed: 32404436
J Virol. 2009 Aug;83(15):7411-21
pubmed: 19439480
PLoS Med. 2006 Jul;3(7):e237
pubmed: 16796401
Nature. 2020 Aug;584(7819):115-119
pubmed: 32454513
EMBO J. 1997 Feb 17;16(4):695-705
pubmed: 9049299
Nat Rev Immunol. 2010 Jul;10(7):514-26
pubmed: 20577268
J Virol. 2013 Mar;87(6):3097-107
pubmed: 23283955
Blood. 2020 Oct 01;:
pubmed: 33001206
Science. 2020 Jun 12;368(6496):1274-1278
pubmed: 32404477
PLoS One. 2013 Aug 30;8(8):e72942
pubmed: 24023659
Nat Commun. 2020 Mar 27;11(1):1620
pubmed: 32221306
Science. 2020 Jul 17;369(6501):330-333
pubmed: 32366695
Cell Host Microbe. 2013 Oct 16;14(4):398-410
pubmed: 24055605
Curr Biol. 2020 Jun 8;30(11):2196-2203.e3
pubmed: 32416074
Cell. 2019 Feb 21;176(5):1026-1039.e15
pubmed: 30712865
Nat Immunol. 2018 Nov;19(11):1179-1188
pubmed: 30333615

Auteurs

Jérémie Prévost (J)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.

Romain Gasser (R)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.

Guillaume Beaudoin-Bussières (G)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.

Jonathan Richard (J)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.

Ralf Duerr (R)

Department of Pathology, New York University School of Medicine, New York, NY 10016, USA.

Annemarie Laumaea (A)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.

Sai Priya Anand (SP)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.

Guillaume Goyette (G)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Mehdi Benlarbi (M)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Shilei Ding (S)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Halima Medjahed (H)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Antoine Lewin (A)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Josée Perreault (J)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Tony Tremblay (T)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Gabrielle Gendron-Lepage (G)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.

Nicolas Gauthier (N)

Hôpital Sacré-Cœur de Montréal, Montreal, QC H4J 1C5, Canada.

Marc Carrier (M)

Hôpital Cité-de-la-Santé, Laval, QC H7M 3L9, Canada.

Diane Marcoux (D)

Hôtel-Dieu de Lévis, Lévis, QC G6V 3Z1, Canada.

Alain Piché (A)

Centre Hospitalier Universitaire de Sherbrooke, Sherbrooke, QC J1H 5H4, Canada.

Myriam Lavoie (M)

CIUSSS du Saguenay-Lac-Saint-Jean, Hôpital de Chicoutimi, Chicoutimi, QC G7H 5H6, Canada.

Alexandre Benoit (A)

Hôpital de Verdun, Montreal, QC H4G 2A3, Canada.

Vilayvong Loungnarath (V)

CHU de Québec, Hôpital Enfant-Jésus, Quebec, QC G1J 1Z4, Canada.

Gino Brochu (G)

CIUSSS de la Mauricie-et-du-Centre-du-Québec, Trois-Rivières, QC G9A 5C5, Canada.

Elie Haddad (E)

Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.
CHU Ste-Justine, Montreal, QC H3T 1C5, Canada.
Département de Pédiatrie, Université de Montréal, Montreal, QC H3T 1C5, Canada.

Hannah D Stacey (HD)

Micheal G. DeGroote Institute for Infectious Disease Research, Master Immunology Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada.

Matthew S Miller (MS)

Micheal G. DeGroote Institute for Infectious Disease Research, Master Immunology Research Centre, Department of Biochemistry and Biomedical Sciences, McMaster University, Hamilton, ON L8N 3Z5, Canada.

Marc Desforges (M)

INRS-Institut Armand Frappier, Laval, QC H7V 1B7, Canada.
CHU Ste-Justine, Montreal, QC H3T 1C5, Canada.

Pierre J Talbot (PJ)

INRS-Institut Armand Frappier, Laval, QC H7V 1B7, Canada.

Graham T Gould Maule (GTG)

Department of Biochemistry, Microbiology and Immunology, and Center for Infection, Immunity, and Inflammation, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Marceline Côté (M)

Department of Biochemistry, Microbiology and Immunology, and Center for Infection, Immunity, and Inflammation, University of Ottawa, Ottawa, ON K1H 8M5, Canada.

Christian Therrien (C)

Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, QC H9X 3R5, Canada.

Bouchra Serhir (B)

Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, QC H9X 3R5, Canada.

Renée Bazin (R)

Héma-Québec, Affaires Médicales et Innovation, Québec, QC G1V 5C3, Canada.

Michel Roger (M)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.
Laboratoire de Santé Publique du Québec, Institut national de santé publique du Québec, Sainte-Anne-de-Bellevue, QC H9X 3R5, Canada.

Andrés Finzi (A)

Centre de Recherche du CHUM, Montreal, QC H2X 0A9, Canada.
Département de Microbiologie, Infectiologie et Immunologie, Université de Montréal, Montreal, QC H2X 0A9, Canada.
Department of Microbiology and Immunology, McGill University, Montreal, QC H3A 2B4, Canada.

Classifications MeSH