Determination of drugs in exhumed liver and brain tissue after over 9 years of burial by liquid chromatography-tandem mass spectrometry-Part 1: Cardiovascular drugs.
LC-MS/MS
brain
cardiovascular drug
exhumation
liver
Journal
Drug testing and analysis
ISSN: 1942-7611
Titre abrégé: Drug Test Anal
Pays: England
ID NLM: 101483449
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
10
07
2020
revised:
17
09
2020
accepted:
25
09
2020
pubmed:
6
10
2020
medline:
29
10
2021
entrez:
5
10
2020
Statut:
ppublish
Résumé
This paper should serve as support for future exhumations in which an analysis of cardiovascular drugs is issued after over 9 years of burial. Amiodarone, amlodipine, atropine, bisoprolol, cafedrine, clonidine, esmolol, furosemide, hydrochlorothiazide, lisinopril, nifedipine, nitrendipine, phenprocoumon, torsemide verapamil, and xipamide were determined in liver and brain tissue of over 100 cases in which exhumation was performed after over 9 years of burial. Diagrams, showing the detectability depending on postmortem period as well as condition of tissues, are presented for furosemide. This paper presents analytical results of cardiovascular drugs that were determined in exhumed liver and brain tissue in a total of 115 cases. Exhumations were executed after 9.5-16.5 years of burial. Analysis was performed by solid phase extraction and liquid chromatography-electrospray mass spectrometry. Bisoprolol, verapamil, and xipamide could be proven in all cases in which these were supposedly administered within a defined time frame. Amiodarone was detectable in over 90%, amlodipine in over 80%, and cafedrine, clonidine, phenprocoumon, and torsemide in over 50% of relevant cases. By contrast, atropine, esmolol, furosemide, hydrochlorothiazide, and lisinopril were found in less than 50% of relevant cases, but nifedipine and nitrendipine were not detectable at all. The percentage of positive results for liver and brain tissue for the relevant administered drugs and corresponding postmortem periods are presented per analyte. Neither time since death nor stage of degradation was a reliable predictor of the success rate, as exemplarily shown for furosemide. The presented data may serve as a reference when deciding whether to exhume a corpse for forensic-toxicological examinations in comparable cases.
Autres résumés
Type: Publisher
(ger)
This paper presents analytical results of cardiovascular drugs that were determined in exhumed liver and brain tissue in a total of 115 cases. Exhumations were executed after 9.5-16.5 years of burial. Analysis was performed by solid phase extraction and liquid chromatography-electrospray mass spectrometry. Bisoprolol, verapamil, and xipamide could be proven in all cases in which these were supposedly administered within a defined time frame. Amiodarone was detectable in over 90%, amlodipine in over 80%, and cafedrine, clonidine, phenprocoumon, and torsemide in over 50% of relevant cases. By contrast, atropine, esmolol, furosemide, hydrochlorothiazide, and lisinopril were found in less than 50% of relevant cases, but nifedipine and nitrendipine were not detectable at all. The percentage of positive results for liver and brain tissue for the relevant administered drugs and corresponding postmortem periods are presented per analyte. Neither time since death nor stage of degradation was a reliable predictor of the success rate, as exemplarily shown for furosemide. The presented data may serve as a reference when deciding whether to exhume a corpse for forensic-toxicological examinations in comparable cases.
Substances chimiques
Cardiovascular Agents
0
Types de publication
Journal Article
Validation Study
Langues
eng
Sous-ensembles de citation
IM
Pagination
595-603Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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