Application, Verification, and Implementation of SARS-CoV-2 Serologic Assays with Emergency Use Authorization.


Journal

Journal of clinical microbiology
ISSN: 1098-660X
Titre abrégé: J Clin Microbiol
Pays: United States
ID NLM: 7505564

Informations de publication

Date de publication:
17 12 2020
Historique:
pubmed: 7 10 2020
medline: 29 12 2020
entrez: 6 10 2020
Statut: epublish

Résumé

Interest continues to grow regarding the role of serologic assays for the detection of prior infection with severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). The U.S. Food and Drug Administration (FDA) has granted emergency use authorization (EUA) status to many SARS-CoV-2 serologic assays. In this document, expert recommendations from clinical microbiologist members of the American Society for Microbiology (ASM) concerning detailed verification strategies for SARS-CoV-2 serologic assays with FDA EUA are provided, as are insights into assay limitations and reporting considerations for laboratories. Assessments concerning single-antibody and multiantibody isotype detection assays, which may provide either differentiated or nondifferentiated (i.e., total antibody) antibody class results, are addressed. Additional considerations prior to assay implementation are also discussed, including biosafety, quality control, and proficiency testing strategies. As the landscape of SARS-CoV-2 serologic testing is rapidly changing, this document provides updated guidance for laboratorians on application of these assays.

Identifiants

pubmed: 33020185
pii: JCM.02148-20
doi: 10.1128/JCM.02148-20
pmc: PMC7771455
pii:
doi:

Substances chimiques

Antibodies, Neutralizing 0
Antibodies, Viral 0

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Informations de copyright

Copyright © 2020 American Society for Microbiology.

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Auteurs

Elitza S Theel (ES)

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA.

Marc Roger Couturier (MR)

Department of Pathology, University of Utah School of Medicine, Salt Lake City, Utah, USA.
ARUP Laboratories, Salt Lake City, Utah, USA.

Laura Filkins (L)

Department of Pathology, University of Texas Southwestern Medical Center, Dallas, Texas, USA.

Elizabeth Palavecino (E)

Department of Pathology, Wake Forest Baptist Medical Center, Winston-Salem, North Carolina, USA.

Stephanie Mitchell (S)

Department of Pathology, University of Pittsburgh School of Medicine, Pittsburgh, Pennsylvania, USA.

Sheldon Campbell (S)

Department of Laboratory Medicine, Yale School of Medicine, West Haven, Connecticut, USA.
Department of Pathology and Laboratory Medicine, VA Connecticut Health Care, West Haven, Connecticut, USA.

Michael Pentella (M)

College of Public Health, University of Iowa, Iowa City, Iowa, USA.
State Hygienic Laboratory, University of Iowa, Coralville, Iowa, USA.

Susan Butler-Wu (S)

Department of Pathology, Keck School of Medicine of USC, Los Angeles, California, USA.

Kurt Jerke (K)

Department of Pathology and Area Laboratory Services, Madigan Army Medical Center, Joint Base Lewis-McChord, Washington, USA.

Vaishali Dharmarha (V)

American Society for Microbiology, Washington, DC, USA.

Peggy McNult (P)

American Society for Microbiology, Washington, DC, USA.

Audrey N Schuetz (AN)

Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota, USA schuetz.audrey@mayo.edu.

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