Insights into Species Preservation: Cryobanking of Rabbit Somatic and Pluripotent Stem Cells.
cryobanking
dimethyl sulfoxide
pluripotent stem cell
rabbit
somatic cell
synthetic medium
Journal
International journal of molecular sciences
ISSN: 1422-0067
Titre abrégé: Int J Mol Sci
Pays: Switzerland
ID NLM: 101092791
Informations de publication
Date de publication:
02 Oct 2020
02 Oct 2020
Historique:
received:
01
08
2020
revised:
18
09
2020
accepted:
28
09
2020
entrez:
7
10
2020
pubmed:
8
10
2020
medline:
27
2
2021
Statut:
epublish
Résumé
Induced pluripotent stem cells (iPSCs) are obtained by genetically reprogramming adult somatic cells via the overexpression of specific pluripotent genes. The resulting cells possess the same differentiation properties as blastocyst-stage embryonic stem cells (ESCs) and can be used to produce new individuals by embryonic complementation, nuclear transfer cloning, or in vitro fertilization after differentiation into male or female gametes. Therefore, iPSCs are highly valuable for preserving biodiversity and, together with somatic cells, can enlarge the pool of reproductive samples for cryobanking. In this study, we subjected rabbit iPSCs (rbiPSCs) and rabbit ear tissues to several cryopreservation conditions with the aim of defining safe and non-toxic slow-freezing protocols. We compared a commercial synthetic medium (STEM ALPHA.CRYO3) with a biological medium based on fetal bovine serum (FBS) together with low (0-5%) and high (10%) concentrations of dimethyl sulfoxide (DMSO). Our data demonstrated the efficacy of a CRYO3-based medium containing 4% DMSO for the cryopreservation of skin tissues and rbiPSCs. Specifically, this medium provided similar or even better biological results than the commonly used freezing medium composed of FBS and 10% DMSO. The results of this study therefore represent an encouraging first step towards the use of iPSCs for species preservation.
Identifiants
pubmed: 33023104
pii: ijms21197285
doi: 10.3390/ijms21197285
pmc: PMC7582889
pii:
doi:
Substances chimiques
Cryoprotective Agents
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : Agence Nationale de la Recherche
ID : ANR-12-RPIB-0013; ANR-11-INBS-0003; ANR-11-INBS-0009; ANR-10-LABX-73; ANR-10-LABX-0061
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