Millisecond dynamics of an unlabeled amino acid transporter.


Journal

Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555

Informations de publication

Date de publication:
06 10 2020
Historique:
received: 06 04 2020
accepted: 16 09 2020
entrez: 7 10 2020
pubmed: 8 10 2020
medline: 30 10 2020
Statut: epublish

Résumé

Excitatory amino acid transporters (EAATs) are important in many physiological processes and crucial for the removal of excitatory amino acids from the synaptic cleft. Here, we develop and apply high-speed atomic force microscopy line-scanning (HS-AFM-LS) combined with automated state assignment and transition analysis for the determination of transport dynamics of unlabeled membrane-reconstituted Glt

Identifiants

pubmed: 33024106
doi: 10.1038/s41467-020-18811-z
pii: 10.1038/s41467-020-18811-z
pmc: PMC7538599
doi:

Substances chimiques

Amino Acid Transport Systems 0
Archaeal Proteins 0
Protein Subunits 0

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, Non-U.S. Gov't Video-Audio Media

Langues

eng

Sous-ensembles de citation

IM

Pagination

5016

Subventions

Organisme : NINDS NIH HHS
ID : R01 NS110790
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States

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Auteurs

Tina R Matin (TR)

Department of Anesthesiology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.
Department of Physiology and Biophysics, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.

George R Heath (GR)

Department of Anesthesiology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.
Department of Physiology and Biophysics, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.

Gerard H M Huysmans (GHM)

Department of Physiology and Biophysics, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.

Olga Boudker (O)

Department of Physiology and Biophysics, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA.
Howard Hughes Medical Institute, Weill Cornell Medicine, New York, NY, 10065, USA.

Simon Scheuring (S)

Department of Anesthesiology, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA. sis2019@med.cornell.edu.
Department of Physiology and Biophysics, Weill Cornell Medicine, 1300 York Avenue, New York, NY, 10065, USA. sis2019@med.cornell.edu.

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