Millisecond dynamics of an unlabeled amino acid transporter.
Journal
Nature communications
ISSN: 2041-1723
Titre abrégé: Nat Commun
Pays: England
ID NLM: 101528555
Informations de publication
Date de publication:
06 10 2020
06 10 2020
Historique:
received:
06
04
2020
accepted:
16
09
2020
entrez:
7
10
2020
pubmed:
8
10
2020
medline:
30
10
2020
Statut:
epublish
Résumé
Excitatory amino acid transporters (EAATs) are important in many physiological processes and crucial for the removal of excitatory amino acids from the synaptic cleft. Here, we develop and apply high-speed atomic force microscopy line-scanning (HS-AFM-LS) combined with automated state assignment and transition analysis for the determination of transport dynamics of unlabeled membrane-reconstituted Glt
Identifiants
pubmed: 33024106
doi: 10.1038/s41467-020-18811-z
pii: 10.1038/s41467-020-18811-z
pmc: PMC7538599
doi:
Substances chimiques
Amino Acid Transport Systems
0
Archaeal Proteins
0
Protein Subunits
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Video-Audio Media
Langues
eng
Sous-ensembles de citation
IM
Pagination
5016Subventions
Organisme : NINDS NIH HHS
ID : R01 NS110790
Pays : United States
Organisme : Howard Hughes Medical Institute
Pays : United States
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