Impact of rs174537 on Critically Ill Patients with Acute Lung Injury: A Secondary Analysis of the OMEGA Randomized Clinical Trial.
ARDS
DHA
EPA
FADS
GLA
OMEGA trial
PUFA
SNP–diet interactions
omega-3
rs174537
Journal
Current developments in nutrition
ISSN: 2475-2991
Titre abrégé: Curr Dev Nutr
Pays: United States
ID NLM: 101717957
Informations de publication
Date de publication:
Oct 2020
Oct 2020
Historique:
received:
08
06
2020
revised:
26
08
2020
accepted:
02
09
2020
entrez:
7
10
2020
pubmed:
8
10
2020
medline:
8
10
2020
Statut:
epublish
Résumé
Nutrition in the intensive care unit is vital for patient care; however, immunomodulatory diets rich in PUFAs like γ-linolenic acid (GLA), EPA, and DHA remain controversial for patients with acute respiratory distress syndrome. We postulate that genetic variants impacting PUFA metabolism contribute to mixed responses to PUFA-rich diets. In this study, we aimed to test the effects of single nucleotide polymorphism (SNP) rs174537 on differential responses to PUFA-rich diets. We performed a secondary analysis of the OMEGA trial (NCT00609180) where 129 subjects received placebo control diets and 143 received omega-oil. DNA was extracted from buffy coats and used to genotype rs174537; plasma was used to quantitate PUFAs. We tested for SNP-diet interactions on PUFA concentrations, inflammatory biomarkers, and patient outcomes. We observed that all individuals receiving omega-oil displayed significantly higher concentrations of GLA, EPA, and DHA (all This study highlights the importance of genetic and racial contributions to PUFA metabolism and inflammation. In particular, rs174537 had a significant impact on circulating DHA and urinary isoprostane concentrations. Given our relatively small sample size, further investigations in larger multiethnic cohorts are needed to evaluate the impact of rs174537 on fatty acid metabolism and downstream inflammation.
Sections du résumé
BACKGROUND
BACKGROUND
Nutrition in the intensive care unit is vital for patient care; however, immunomodulatory diets rich in PUFAs like γ-linolenic acid (GLA), EPA, and DHA remain controversial for patients with acute respiratory distress syndrome. We postulate that genetic variants impacting PUFA metabolism contribute to mixed responses to PUFA-rich diets.
OBJECTIVES
OBJECTIVE
In this study, we aimed to test the effects of single nucleotide polymorphism (SNP) rs174537 on differential responses to PUFA-rich diets.
METHODS
METHODS
We performed a secondary analysis of the OMEGA trial (NCT00609180) where 129 subjects received placebo control diets and 143 received omega-oil. DNA was extracted from buffy coats and used to genotype rs174537; plasma was used to quantitate PUFAs. We tested for SNP-diet interactions on PUFA concentrations, inflammatory biomarkers, and patient outcomes.
RESULTS
RESULTS
We observed that all individuals receiving omega-oil displayed significantly higher concentrations of GLA, EPA, and DHA (all
CONCLUSIONS
CONCLUSIONS
This study highlights the importance of genetic and racial contributions to PUFA metabolism and inflammation. In particular, rs174537 had a significant impact on circulating DHA and urinary isoprostane concentrations. Given our relatively small sample size, further investigations in larger multiethnic cohorts are needed to evaluate the impact of rs174537 on fatty acid metabolism and downstream inflammation.
Identifiants
pubmed: 33024925
doi: 10.1093/cdn/nzaa147
pii: nzaa147
pmc: PMC7524639
doi:
Types de publication
Journal Article
Langues
eng
Pagination
nzaa147Subventions
Organisme : NHLBI NIH HHS
ID : K25 HL133611
Pays : United States
Organisme : NCCIH NIH HHS
ID : R01 AT008621
Pays : United States
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of American Society for Nutrition.
Références
Crit Care Med. 1999 Aug;27(8):1409-20
pubmed: 10470743
Curr Opin Clin Nutr Metab Care. 2016 Mar;19(2):103-10
pubmed: 26828581
N Engl J Med. 1985 May 9;312(19):1217-24
pubmed: 2985986
PLoS Genet. 2009 Jan;5(1):e1000338
pubmed: 19148276
PLoS One. 2014 May 19;9(5):e97510
pubmed: 24842322
Crit Care Med. 2006 Sep;34(9):2325-33
pubmed: 16850002
BMC Genet. 2011 May 20;12:50
pubmed: 21599946
N Engl J Med. 2019 Jan 3;380(1):11-22
pubmed: 30415628
Stat Med. 2000 Jul 15;19(13):1793-819
pubmed: 10861779
JPEN J Parenter Enteral Nutr. 2005 Jan-Feb;29(1 Suppl):S62-9
pubmed: 15709547
Br J Nutr. 2012 Feb;107(4):547-55
pubmed: 21733300
Nature. 2014 Jun 5;510(7503):92-101
pubmed: 24899309
Cochrane Database Syst Rev. 2019 Jan 24;1:CD012041
pubmed: 30677127
Chest. 2005 Aug;128(2):525-32
pubmed: 16100134
Am J Clin Nutr. 2020 May 1;111(5):1068-1078
pubmed: 32167131
JAMA. 2016 Feb 23;315(8):788-800
pubmed: 26903337
Crit Care Med. 2006 Apr;34(4):1033-8
pubmed: 16484911
Semin Respir Crit Care Med. 2019 Feb;40(1):57-65
pubmed: 31060088
PLoS One. 2013;8(1):e55869
pubmed: 23383292
Sci Rep. 2017 Nov 16;7(1):15724
pubmed: 29146976
Nutrients. 2017 Oct 25;9(11):
pubmed: 29068398
Am J Clin Nutr. 2000 Jan;71(1 Suppl):393S-6S
pubmed: 10618003
JAMA. 2011 Oct 12;306(14):1574-81
pubmed: 21976613
Genes Nutr. 2019 Jun 18;14:20
pubmed: 31244960
Genes Nutr. 2014 Nov;9(6):437
pubmed: 25367143
J Biol Chem. 2014 Aug 8;289(32):22482-9
pubmed: 24962583
J Lipid Res. 2010 Nov;51(11):3281-8
pubmed: 20802161
Clin Interv Aging. 2013;8:585-96
pubmed: 23818766
Ann Transl Med. 2017 Jul;5(14):283
pubmed: 28828358
F1000Res. 2018 Mar 5;7:
pubmed: 29568488
Nutrients. 2015 Dec 03;7(12):10100-15
pubmed: 26633493
FASEB J. 2020 Jun 24;:
pubmed: 32579292
Crit Care Med. 2003 Feb;31(2):491-500
pubmed: 12576957
Am J Clin Nutr. 2006 Jun;83(6 Suppl):1505S-1519S
pubmed: 16841861