Occurrence of a human-associated microbial source tracking marker and its relationship with faecal indicator bacteria in an urban estuary.
Golden Horn (Turkey)
faecal indicator bacteria
human-associated MST marker (BT-α)
microbial source tracking
quantitative polymerase chain reaction
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
28
07
2020
revised:
20
09
2020
accepted:
29
09
2020
pubmed:
8
10
2020
medline:
17
3
2021
entrez:
7
10
2020
Statut:
ppublish
Résumé
One of the main impacts of urban sprawl in rapidly growing countries has been contamination of coastal environments by waterborne pathogens, posing a critical risk to ecosystem and human health. Microbial source tracking (MST) has been a robust tool to identify the origin of these pathogens globally. This study compared the occurrence of a human-associated Bacteroides marker (BT-α) with faecal indicator bacteria (FIB) in an urban estuary (Golden Horn, Istanbul, Turkey). Faecal coliform (culture method), enterococci (both culture and qPCR method) concentrations and physicochemical variables were compared with the BT-α concentrations in monthly collected samples for a year (n = 108). Enterococci concentrations detected by culture and qPCR were positively correlated (r = 0·86, P < 0·01) suggesting that qPCR can be an alternative method for monitoring. BT-α marker was positive for 30% of the samples and positively correlated with enterococci (r = 0·61 and r = 0·64 for culture and qPCR methods respectively, P < 0·01). Rainfall had a moderate positive correlation with all faecal/MST indicators suggesting combined sewer overflows also severely impacted estuarine water quality. The high FIB and BT-α concentrations at upper estuary suggested that faecal pollution mainly originated from the peri-urban settlements around two creeks entering the estuary.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
167-177Subventions
Organisme : Bilimsel Araştirma Projeleri Birimi, Istanbul Üniversitesi
ID : 11659
Organisme : Istanbul University
ID : 11659
Informations de copyright
© 2020 The Society for Applied Microbiology.
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