Fluorescence lifetime metabolic mapping of hypoxia-induced damage in pancreatic pseudo-islets.
FLIM
hypoxia
insulin
pancreatic islet
type 1 diabetes
Journal
Journal of biophotonics
ISSN: 1864-0648
Titre abrégé: J Biophotonics
Pays: Germany
ID NLM: 101318567
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
16
09
2020
revised:
03
10
2020
accepted:
04
10
2020
pubmed:
8
10
2020
medline:
24
6
2021
entrez:
7
10
2020
Statut:
ppublish
Résumé
Pancreatic islet isolation from donor pancreases is an essential step for the transplantation of insulin-secreting β-cells as a therapy to treat type 1 diabetes mellitus. This process however damages islet basement membranes, which can lead to islet dysfunction or death. Posttransplantation, islets are further stressed by a hypoxic environment and immune reactions that cause poor engraftment and graft failure. The current standards to assess islet quality before transplantation are destructive procedures, performed on a small islet population that does not reflect the heterogeneity of large isolated islet batches. In this study, we incorporated fluorescence lifetime imaging microscopy (FLIM) into a pancreas-on-chip system to establish a protocol to noninvasively assess the viability and functionality of pancreatic β-cells in a three-dimensional in vitro model (= pseudo-islets). We demonstrate how (pre-) hypoxic β-cell-composed pseudo-islets can be discriminated from healthy functional pseudo-islets according to their FLIM-based metabolic profiles. The use of FLIM during the pretransplantation pancreatic islet selection process has the potential to improve the outcome of β-cell islet transplantation.
Identifiants
pubmed: 33026180
doi: 10.1002/jbio.202000375
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
e202000375Informations de copyright
© 2020 The Authors. Journal of Biophotonics published by Wiley-VCH GmbH.
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