Establishment of a fluorescence staining method for Schistosoma japonicum miracidia.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
07 10 2020
07 10 2020
Historique:
received:
08
05
2020
accepted:
16
09
2020
entrez:
8
10
2020
pubmed:
9
10
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Currently the diagnosis of schistosomiasis is mainly determined by observing the presence of eggs in host stool samples. Because of the overwhelming number of impurities in the stool, eggs are rarely observed. Therefore, the stool hatching method is used to observe the miracidia in the water. However, the miracidia of Schistosoma japonicum are small and difficult to detect, and missed detection is likely to occur when the infection level is low. In this study, recombinant streptococcal protein G-enhanced green fluorescent protein (rSPG-EGFP) was expressed, purified, and used as a fluorescence staining reagent for miracidia. A preliminary miracidium fluorescence staining method based on antigen and antibody bindingwas established by combining recombinant protein staining with the stool hatching method. The stool hatching method was used to collect the miracidia of S. japonicum, and Schistosoma-positive serum and the recombinant protein were mixed to assess the feasibility of fluorescence staining of miracidia. The miracidia of S. japonicum and Schistosoma turkestanicum were incubated with S. japonicum-positive serum and S. turkestanicum-positive serum, respectively, to identify miracidia species. When the fluorescence staining method was used to observe living miracidia, the miracidiawere labelled by the recombinant protein, and their motility status was not affected.
Identifiants
pubmed: 33028866
doi: 10.1038/s41598-020-73526-x
pii: 10.1038/s41598-020-73526-x
pmc: PMC7542450
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
16766Références
Hotez, P. J. et al. Helminth infections: the great neglected tropical diseases. J. Clin. Investig. 118, 1311–1321 (2008).
doi: 10.1172/JCI34261
Gryseels, B., Polman, K., Clerinx, J. & Kestens, L. Human schistosomiasis. Lancet 368, 1106–1118 (2006).
doi: 10.1016/S0140-6736(06)69440-3
Guo, J. The history and present situation of comprehensive control on schistosomiasis in China. Chin. J. Prev. Med. 40, 225–228 (2006).
Chen, H. et al. Study on comprehensive schistosomiasis control strategy with emphasis on infectious source control in Poyang Lake areas. Chin. J. Schistosomiasis Control 21, 243–249 (2009).
Wu, G. L. The review the prospect of the development of immune diagnosis of schistosomiasis in China. Chin. J. Parasitol. Parasit. Dis. 23, 323–328 (2005).
Jin, J. N. et al. Endemic situation at schistosomiasis surveillance sites in China in 2016. Chin. J. Parasitol. Parasit. Dis. 35, 542–548 (2017).
Zhu, R. et al. Assessment of effect and quality control for parasitological tests in national schistosomiasis surveillance sites. Chin. J. Schistosomiasis Control 25, 11–15 (2013).
Khalil, H. M., Seitz, H. M., Makled, M. K., El Missiry, A. G. & Serougi, A. O. A comparative study between IFAT, COPT, CHR and IDT in the diagnosis of schistosomiasis mansoni. J. Egypt. Soc. Parasitol. 19, 13–20 (1989).
pubmed: 2496176
Liu, C. S., Li, P. S., Wu, C. Y. & Yang, T. C. The value of cercariae membrane reaction in the diagnosis of schistosomiasis japonica. Chin. Med. J. 77, 136–143 (1958).
pubmed: 13585540
Spencer, L., De Noya, B. A., Noya, O. & Masroua, G. Comparative analysis between the circumoval precipitin test and ELISA with raw antigens for the diagnosis of schistosomiasis in Venezuela. G E N45, 77–83 (1991).
Kutsumi, H., Minai, M. & Kajihara, N. Studies on the diagnosis of schistosomiasis japonica. (2) analysis of the efficiency of MIFC method on the detection of schistosome eggs immersed into feces (author’s transl). Hokkaido Igaku Zasshi 54, 347–353 (1979).
pubmed: 527924
Björck, L. & Kronvall, G. Purification and some properties of streptococcal protein G, a novel IgG-binding reagent. J. Immunol. 133, 969–974 (1984).
pubmed: 6234364
Kobatake, E., Nishimori, Y., Ikariyama, Y., Aizawa, M. & Kato, S. Application of a fusion protein, metapyrocatechase/protein A, to an enzyme immunoassay. Anal. Biochem. 186, 14–18 (1990).
doi: 10.1016/0003-2697(90)90564-P
Engels, D., Chitsulo, L., Montresor, A. & Savioli, L. The global epidemiological situation of schistosomiasis and new approaches to control and research. Acta Trop. 82, 139–146 (2002).
doi: 10.1016/S0001-706X(02)00045-1
Li-Juan, Z. et al. Endemic status of schistosomiasis in People’s Republic of China in 2017. Chin. J. Schistosomiasis Control 30, 481–488 (2018).
Song, L. G., Wu, X. Y., Sacko, M. & Wu, Z. D. History of schistosomiasis epidemiology, current status, and challenges in China: on the road to schistosomiasis elimination. Parasitol. Res. 115, 4071–4081 (2016).
doi: 10.1007/s00436-016-5253-5
Lan, W. et al. Parallel detection of the circulating antigens and antibodies in the diagnosis of schistosomiasis. Chin. J. Zoonoses 25, 1218–1221 (2009).
Gu, Q., Zhang, H., Zhu, S. & Xu, W. Preliminary studies on microwave irradiation ELISA for diagnosis of schistosomiasis japonica. Chin. J. Parasitol. Parasit. Dis. 17, 115–116 (1999).
Jie, Z. et al. Study on value of IHA in diagnosis of schistosomiasis japonica. Chin. J. Schistosomiasis Control 28, 375–380 (2016).
Lin, D. D. et al. Evaluation on application of common diagnosis methods for schistosomiasis japonica in endemic areas of China. III. Analysis and evaluation of underestimation of prevalence of Schistosoma japonicum infection by routine Kato-Katz technique. Chin. J. Schistosomiasis Control 23, 642–647 (2011).
Zhu, M., Zhong, C. & Hong, L. Rapid diagnosis for schistosomiasis by colloidal gold immunochromatographic assay. Chin. J. Zoonoses 26, 942–943 (2010).
Xu, R. et al. A novel colloidal gold immunochromatography assay strip for the diagnosis of schistosomiasis japonica in domestic animals. Infect. Dis. Poverty 6, 84 (2017).
doi: 10.1186/s40249-017-0297-z
Xu, B., Feng, Z., Xu, X. J. & Hu, W. Evaluation of Kato-Katz technique combined with stool hatching test in diagnosis of schistosomiasis japonica. Chin. J. Schistosomiasis Control 23, 321–323 (2011).
Jurberg, A. D., Oliveira, A. A., Lenzi, H. L. & Coelho, P. M. A new miracidia hatching device for diagnosing schistosomiasis. Mem. Inst. Oswaldo Cruz 103, 112–114 (2008).
doi: 10.1590/S0074-02762008005000005
Dutt, S. C. & Srivastava, H. D. A revision of the genus Ornithobilharzia Odhner, 1912 (Trematoda: Schistosomatidae) in Proceedings of the 42nd Indian Science Congress, 285 (ISC, 1955).
Morand, S. & Müller-Graf, C. Muscles or testes? Comparative evidence for sexual competition among dioecious blood parasites (Schistosomatidae) of vertebrates. Parasitology 120, 45–56 (2000).
doi: 10.1017/S0031182099005235
Yuan, G., Pei-Cai, Y., Su, Y. & Fei, X. Effectiveness evaluation of dynamic automatic identification system (device) of Schistosoma japonicum miracidia. Chin. J. Schistosomiasis Control 30, 54–56 (2018).
Pei-Cai, Y. et al. Detection effect of an automatic identification system of Schistosoma japonicum miracidia. Chin. J. Schistosomiasis Control 30, 433–435 (2018).
Chen, J. et al. Human Parasitology (ed. Chen, J.) 138–156 (Sichuan University Press, 2015).