Regeneration of Rat Sciatic Nerve Using PLGA Conduit Containing Rat ADSCs with Controlled Release of BDNF and Gold Nanoparticles.
Adipose Tissue
/ cytology
Animals
Brain-Derived Neurotrophic Factor
/ administration & dosage
Cells, Cultured
Chitosan
/ chemistry
Drug Liberation
Gold
/ chemistry
Guided Tissue Regeneration
/ methods
Laminin
/ chemistry
Male
Mesenchymal Stem Cell Transplantation
/ methods
Mesenchymal Stem Cells
/ cytology
Metal Nanoparticles
/ chemistry
Nerve Regeneration
Peripheral Nerve Injuries
/ therapy
Polylactic Acid-Polyglycolic Acid Copolymer
/ chemistry
Rats
Rats, Wistar
Sciatic Nerve
/ injuries
Tissue Scaffolds
/ chemistry
Adipose-derived stem cells
Brain-derived neurotrophic factor
Controlled release
Gold nanoparticle
Nerve conduit
Poly(L-lactide-co-glycolide)
Journal
Journal of molecular neuroscience : MN
ISSN: 1559-1166
Titre abrégé: J Mol Neurosci
Pays: United States
ID NLM: 9002991
Informations de publication
Date de publication:
Apr 2021
Apr 2021
Historique:
received:
04
07
2020
accepted:
28
08
2020
pubmed:
9
10
2020
medline:
28
10
2021
entrez:
8
10
2020
Statut:
ppublish
Résumé
Implantation of a nerve guidance conduit (NGC) carrying neuroprotective factors is promising for repairing peripheral nerve injury. Here, we developed a novel strategy for repairing peripheral nerve injury by gold nanoparticles (AuNPs) and brain-derived neurotrophic factor (BDNF)-encapsulated chitosan in laminin-coated nanofiber of Poly(l-lactide-co-glycolide) (PLGA) conduit and transplantation of rat adipose-derived stem cells (r-ADSCs) suspended in alginate. Then, the beneficial effect of AuNPs, BDNF, and r-ADSCs on nerve regeneration was evaluated in rat sciatic nerve transection model. In vivo experiments showed that the combination of AuNPs- and BDNF-encapsulated chitosan nanoparticles in laminin-coated nanofiber of PLGA conduit with r-ADSCs could synergistically facilitate nerve regeneration. Furthermore, the in vivo histology, immunohistochemistry, and behavioral results demonstrated that the AuNPs- and BDNF-encapsulated chitosan nanoparticles in NGC could significantly reinforce the repair performance of r-ADSCs, which may also contribute to the therapeutic outcome of the AuNPs, BDNF, and r-ADSCs strategies. In this study, we found that the combination of AuNPs and BDNF releases in NGC with r-ADSCs may represent a new potential strategy for peripheral nerve regeneration.
Identifiants
pubmed: 33029736
doi: 10.1007/s12031-020-01694-6
pii: 10.1007/s12031-020-01694-6
doi:
Substances chimiques
Brain-Derived Neurotrophic Factor
0
Laminin
0
Polylactic Acid-Polyglycolic Acid Copolymer
1SIA8062RS
Gold
7440-57-5
Chitosan
9012-76-4
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
746-760Références
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