The Anti-oxidative Effects of Encapsulated Cysteamine During Mice In Vitro Matured Oocyte/Morula-Compact Stage Embryo Culture Model: a Comparison of High-Efficiency Nanocarriers for Hydrophilic Drug Delivery-a Pilot Study.
Animals
Antioxidants
/ administration & dosage
Cysteamine
/ administration & dosage
Drug Delivery Systems
/ methods
Embryonic Development
/ drug effects
Female
Hydrophobic and Hydrophilic Interactions
Mice
Morula
/ drug effects
Nanocapsules
Oocytes
/ drug effects
Pilot Projects
Reproductive Techniques, Assisted
Cysteamine
Hydrophilic molecule delivery
Intra-/extracellular ROS level
Mouse oocyte/embryo
Nanoparticles chitosan
SLN based on self-emulsifying lipids
Journal
Reproductive sciences (Thousand Oaks, Calif.)
ISSN: 1933-7205
Titre abrégé: Reprod Sci
Pays: United States
ID NLM: 101291249
Informations de publication
Date de publication:
05 2021
05 2021
Historique:
received:
15
07
2020
accepted:
22
09
2020
pubmed:
9
10
2020
medline:
28
1
2022
entrez:
8
10
2020
Statut:
ppublish
Résumé
Although it is well-recognized that antioxidant nano-encapsulation has many benefits such as minimizing side effects (e.g., high-dose toxicity), the most attention was paid to the hydrophobic antioxidant not hydrophilic. In this regard, we sought to compare two hydrophilic model nanocarriers to deliver the optimal dose of cystamine (Cys) into the in vitro matured oocyte and the first cleavage stages until morula-compact stage embryonic cells. The formation of Cys-loaded solid self-emulsifying lipid (Cys + SLN) and Cys-loaded chitosan shell (Cys-CS-NC) were confirmed by FT-IR and UV-Vis spectrophotometry, dynamic light scattering (DLS), transmission electron microscopy (TEM), and scanning electron microscopy (SEM) technologies. In two experiments, the oocytes/presumptive zygotes were cultured under various concentrations of Cys-SLN and Cys-CS-NC. The results of nuclear staining (aceto-orcein and Hoechst 33342), H2DCFDA fluorescent staining, chemiluminescence test, and quantitative reverse transcription-PCR (qRT-PCR) technique as in vitro toxicity studies demonstrated that adding the lowest dose of Cys-encapsulated in both nanocarriers [Cys-SLN (5 μM) and Cys-CS-NC (10 μM)] to maturation or culture medium could accumulate a strong anti-oxidative effect in oocyte/embryo by controlled release and enhanced intracellular penetration of Cys. In comparison, Cys-SLN (5 μM) is more effective than Cys-CS-NC (10 μM) groups to improve the expression of antioxidant genes (SOD, CAT, GPx) or anti-apoptotic (BCL-2) gene and decreased apoptosis (BAX and caspase-3) or intra-/extracellular ROS levels. In a nutshell, both nanocarriers (CS-NC or SLN) can deliver the lowest dose of Cys into the oocyte/embryo, thus encouraging a better expansion of antioxidant genes and enhancing the development of in vitro oocyte/embryo.
Identifiants
pubmed: 33030694
doi: 10.1007/s43032-020-00333-0
pii: 10.1007/s43032-020-00333-0
doi:
Substances chimiques
Antioxidants
0
Nanocapsules
0
Cysteamine
5UX2SD1KE2
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
1290-1306Références
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