Reduction of the Carbapenemase Inactivation Method (CIM) assay time by real-time PCR.
Antimicrobial resistance
Carbapenemase Inactivation Method (CIM)
PCR
natCIM
Journal
Journal of microbiological methods
ISSN: 1872-8359
Titre abrégé: J Microbiol Methods
Pays: Netherlands
ID NLM: 8306883
Informations de publication
Date de publication:
06 Oct 2020
06 Oct 2020
Historique:
received:
01
09
2020
revised:
29
09
2020
accepted:
30
09
2020
pubmed:
9
10
2020
medline:
9
10
2020
entrez:
8
10
2020
Statut:
aheadofprint
Résumé
Carbapenemase Inactivation Method (CIM) is a test to detect presence of the carbapenemase in Gram-negative bacteria. Determination of the carbapenemase production by inactivation of meropenem requires that a zone of control E. coli inhibition be measured approximately 6-24 h after plating. We have modified the CIM test by developing a rapid method which instead measures the growth of E. coli indicator strain ATCC 25922 using real-time PCR, referred to as a nucleic acid testing CIM (natCIM). Our natCIM, therefore reduces the detecting time from 6 to 24 h to approximately 4 h.
Identifiants
pubmed: 33031896
pii: S0167-7012(20)30788-0
doi: 10.1016/j.mimet.2020.106072
pii:
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
106072Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.
Déclaration de conflit d'intérêts
Declaration of Competing Interest The authors declare that they have no known competing financial interests or personal relationships that could have appeared to influence the work reported in this paper.