TNF-α Induces URG-4/URGCP Gene Expression in Hepatoma Cells through Starvation Dependent Manner.
Apoptosis
Carcinoma, Hepatocellular
/ metabolism
Cell Line, Tumor
Cell Proliferation
Cell Survival
Cytokines
/ metabolism
Gene Expression Regulation, Neoplastic
Humans
Inflammation
Liver Neoplasms
/ metabolism
Neoplasm Proteins
/ genetics
Phosphatidylinositol 3-Kinases
/ metabolism
Transcription, Genetic
Tumor Necrosis Factor-alpha
/ pharmacology
Hep3B
Pathway
Serum induced
Serum starved
TNF-α
URG-4/URGCP
Journal
Biochemical genetics
ISSN: 1573-4927
Titre abrégé: Biochem Genet
Pays: United States
ID NLM: 0126611
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
23
12
2019
accepted:
12
05
2020
pubmed:
10
10
2020
medline:
9
7
2021
entrez:
9
10
2020
Statut:
ppublish
Résumé
URG-4/URGCP is a gene that may be associated with the onset of tumorigenesis and cell cycle regulation. In the literature, there is no study about inflammatory cytokine-mediated URG-4/URGCP regulation. In this study, the effect of TNF-α cytokine was investigated on URG-4/URGCP expression in serum-starved and serum-cultured hepatoma cells. The effect of TNF-α on hepatoma cells was shown using MTT and Annexin-V/PI staining with flow cytometer analyses. As a result, TNF-α leads to the cytotoxicity of hepatoma cells in serum-starved condition whereas no decrease was detected from serum-cultured condition. TNF-α-mediated URG-4/URGCP expression was determined at mRNA and protein level with qRT-PCR analyses and Western blotting method. URG-4URGCP mRNA expression was upregulated in both serum-starved and serum-cultured hepatoma cells. The transfection studies were carried out with URG-4/URGCP promoter constructs for determining the transcriptional activity. TNF-α caused to the upregulation of the activities of URG/URGCP promoter constructs. The basal activities of the URG-4/URGCP promoter conditions are differential according to serum conditions. In addition, some pathway inhibitors were added into hepatoma cells for blocking specific pathways to find out TNF-α-mediated URG-4/URGCP upregulation at mRNA and protein level. TNF-α used JNK and PI3K pathways for regulating URG-4/URGCP gene at serum-starved Hep3B cells. In serum-cultured condition, wortmannin (PI3K inhibitor), MEK-1 (MAPK inhibitor), and SP600125 (JNK inhibitor) did not inhibit the activation response of TNF-α on URGCP.
Identifiants
pubmed: 33034821
doi: 10.1007/s10528-020-09972-z
pii: 10.1007/s10528-020-09972-z
doi:
Substances chimiques
Cytokines
0
Neoplasm Proteins
0
Tumor Necrosis Factor-alpha
0
URGCP protein, human
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
300-314Références
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