Functional analysis and evaluation of respiratory cilia in healthy Chinese children.
Beat frequency
Beat pattern
Chinese children
Nasal cilia
Reference data
Ultrastructure
Journal
Respiratory research
ISSN: 1465-993X
Titre abrégé: Respir Res
Pays: England
ID NLM: 101090633
Informations de publication
Date de publication:
09 Oct 2020
09 Oct 2020
Historique:
received:
09
06
2020
accepted:
10
09
2020
entrez:
10
10
2020
pubmed:
11
10
2020
medline:
6
8
2021
Statut:
epublish
Résumé
To aid in the diagnosis of Primary Ciliary Dyskinesia (PCD) and to evaluate the respiratory epithelium in respiratory disease, normal age-related reference ranges are needed for ciliary beat frequency (CBF), beat pattern and ultrastructure. Our aim was to establish reference ranges for healthy Chinese children. Ciliated epithelial samples were obtained from 135 healthy Chinese children aged below 18 years by brushing the inferior nasal turbinate. CBF and beat pattern were analysed from high speed video recordings. Epithelial integrity and ciliary ultrastructure were assessed using transmission electronic microscopy. The mean CBF from 135 children studied was 10.1 Hz (95% CI 9.8 to 10.4). Approximately 20% (ranged 18.0-24.2%) of ciliated epithelial edges were found to have areas of dyskinetically beating cilia. Normal beat pattern was observed in ciliated epithelium from all subjects. We did not find any effect of exposure to second hand smoke on CBF in our subjects. Microtubular defects were found in 9.3% of all of the cilia counted in these children, while other ciliary ultrastructural defects were found in less than 3%. We established the reference range for CBF, beat pattern and ultrastructure in healthy Chinese children. Using similar methodology, we found a lower overall mean CBF than previously obtained European values. This study highlights the need to establish normative data for ciliary function in different populations.
Sections du résumé
BACKGROUND
BACKGROUND
To aid in the diagnosis of Primary Ciliary Dyskinesia (PCD) and to evaluate the respiratory epithelium in respiratory disease, normal age-related reference ranges are needed for ciliary beat frequency (CBF), beat pattern and ultrastructure. Our aim was to establish reference ranges for healthy Chinese children.
METHODS
METHODS
Ciliated epithelial samples were obtained from 135 healthy Chinese children aged below 18 years by brushing the inferior nasal turbinate. CBF and beat pattern were analysed from high speed video recordings. Epithelial integrity and ciliary ultrastructure were assessed using transmission electronic microscopy.
RESULTS
RESULTS
The mean CBF from 135 children studied was 10.1 Hz (95% CI 9.8 to 10.4). Approximately 20% (ranged 18.0-24.2%) of ciliated epithelial edges were found to have areas of dyskinetically beating cilia. Normal beat pattern was observed in ciliated epithelium from all subjects. We did not find any effect of exposure to second hand smoke on CBF in our subjects. Microtubular defects were found in 9.3% of all of the cilia counted in these children, while other ciliary ultrastructural defects were found in less than 3%.
CONCLUSIONS
CONCLUSIONS
We established the reference range for CBF, beat pattern and ultrastructure in healthy Chinese children. Using similar methodology, we found a lower overall mean CBF than previously obtained European values. This study highlights the need to establish normative data for ciliary function in different populations.
Identifiants
pubmed: 33036612
doi: 10.1186/s12931-020-01506-w
pii: 10.1186/s12931-020-01506-w
pmc: PMC7545929
doi:
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
259Subventions
Organisme : Health and Medical Research Fund
ID : HMRF No.02133316
Références
Thorax. 2003 Apr;58(4):333-8
pubmed: 12668798
Thorax. 2000 Apr;55(4):314-7
pubmed: 10722772
Inhal Toxicol. 2009 Aug;21(10):875-81
pubmed: 19555226
Breathe (Sheff). 2017 Sep;13(3):166-178
pubmed: 28894478
Cells. 2016 Nov 11;5(4):
pubmed: 27845721
Intensive Care Med. 1998 Aug;24(8):791-4
pubmed: 9757922
Sci Rep. 2019 Feb 1;9(1):1400
pubmed: 30710127
J Allergy Clin Immunol. 2010 Oct;126(4):722-729.e2
pubmed: 20673980
Am J Respir Crit Care Med. 2004 Feb 15;169(4):459-67
pubmed: 14656747
J Clin Pathol. 2012 Mar;65(3):267-71
pubmed: 22135026
Chest. 2016 Jun;149(6):1445-59
pubmed: 26836936
Chest. 2017 May;151(5):993-1001
pubmed: 27693596
Am J Respir Crit Care Med. 2018 Jun 15;197(12):e24-e39
pubmed: 29905515
Pediatr Pulmonol. 1992 Dec;14(4):201-5
pubmed: 1484753
Am J Respir Crit Care Med. 2010 Feb 15;181(4):307-14
pubmed: 19910612
Eur Respir J. 2001 Dec;18(6):965-70
pubmed: 11829103
Thorax. 2013 Feb;68(2):190-1
pubmed: 23192883
Clin Otolaryngol Allied Sci. 1998 Jun;23(3):227-30
pubmed: 9669071
Chest. 2010 Dec;138(6):1441-7
pubmed: 20616212
J Allergy Clin Immunol. 2003 Sep;112(3):518-24
pubmed: 13679810
Orphanet J Rare Dis. 2012 Oct 11;7:78
pubmed: 23057704
Eur Arch Otorhinolaryngol. 2010 Sep;267(9):1383-7
pubmed: 20169353
In Vitro Cell Dev Biol Anim. 2010 Jul;46(7):606-12
pubmed: 20383665
Eur Respir J. 2016 Mar;47(3):837-48
pubmed: 26647444
N Engl J Med. 1985 Feb 21;312(8):463-8
pubmed: 3969108
PLoS One. 2014 Feb 25;9(2):e89675
pubmed: 24586956
Epidemiol Infect. 2016 Jun;144(8):1622-33
pubmed: 26732801
Am J Respir Crit Care Med. 2004 Mar 1;169(5):634-7
pubmed: 14982824
Eur Respir J. 1997 Oct;10(10):2376-9
pubmed: 9387968
Eur Respir J. 2019 Sep 5;54(3):
pubmed: 31488586
Inhal Toxicol. 2017 Feb;29(3):137-144
pubmed: 28470140
BMC Public Health. 2016 Mar 01;16:202
pubmed: 26932396
Acta Otorhinolaryngol Belg. 2000;54(3):325-32
pubmed: 11082769