High-throughput evolutionary optimization of the induction medium towards recombinant protein production in BY-2 tobacco.
bioprocess optimization
combinatorial screening
gene game
genetic algorithms
induct X
Journal
Biotechnology and bioengineering
ISSN: 1097-0290
Titre abrégé: Biotechnol Bioeng
Pays: United States
ID NLM: 7502021
Informations de publication
Date de publication:
02 2021
02 2021
Historique:
received:
13
08
2020
revised:
05
10
2020
accepted:
09
10
2020
pubmed:
11
10
2020
medline:
26
11
2021
entrez:
10
10
2020
Statut:
ppublish
Résumé
Bright yellow (BY-2) tobacco cells combined with the XVE chemically inducible system are one of the most promising plant-based platforms for recombinant protein production. This offers a range of benefits, including the separation of the cell growth and heterologous gene expression, lack of risk of infecting the end product with prions and human viruses or appropriate protein glycosylation and folding. However, low protein productivity remains a major obstacle that limits the extensive commercialization of bioproduction in plants. A number of molecular, cell culture and down processing approaches have been made to overcome this problem. Media development for the specific nutritional and hormonal requirements of transgenic plant cells is one of the most efficient cell-culture approaches. We optimized the induction medium towards recombinant protein production in BY-2 and demonstrated the usefulness of evolutionary medium optimization for high-yield protein production in liquid plant cultures. A reliable XVE/GFP model, parallel conducting experiments in a microscale on 96-well plates, and dedicated Gene Game evolutionary optimization software allowed for an effective search of 76
Substances chimiques
Culture Media
0
Recombinant Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
676-689Subventions
Organisme : National Science Centre (NCN) of Poland
ID : DEC-2013/09/D/NZ1/03364
Organisme : National Centre for Research and Development (NCBR) of Poland
ID : STRATEGMED1/233624/5/NCBR/2014
Informations de copyright
© 2020 Wiley Periodicals LLC.
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