Cytotoxicity and apoptosis induction by Fumaria officinalis extracts in leukemia and multiple myeloma cell lines.
Antineoplastic Agents, Phytogenic
/ administration & dosage
Apoptosis
/ drug effects
Autophagy
/ drug effects
Cell Line, Tumor
Fumaria
/ chemistry
Humans
Inhibitory Concentration 50
Leukemia
/ drug therapy
Membrane Potential, Mitochondrial
/ drug effects
Multiple Myeloma
/ drug therapy
Plant Extracts
/ administration & dosage
Reactive Oxygen Species
/ metabolism
Apoptosis
Autophagy
Ferroptosis
Fumaria officinalis
Multiple myeloma
Phytotherapy
Journal
Journal of ethnopharmacology
ISSN: 1872-7573
Titre abrégé: J Ethnopharmacol
Pays: Ireland
ID NLM: 7903310
Informations de publication
Date de publication:
10 Feb 2021
10 Feb 2021
Historique:
received:
14
08
2020
revised:
30
09
2020
accepted:
03
10
2020
pubmed:
12
10
2020
medline:
26
2
2021
entrez:
11
10
2020
Statut:
ppublish
Résumé
Fumaria officinalis (Fumariaceae) is recorded in the Kurdish ethnobotany for various health problems. In this study, the cytotoxic activity of F. officinalis extracts on two leukemia and nine multiple myeloma (MM) cell lines was investigated. The cytotoxic and ferroptotic activity were examined by resazurin reduction assay. Flow cytometry, immunoblotting assay and fluorescence microscopy were used to measure cell cycle distribution, apoptosis, induction of reactive oxygen species (ROS), loss integrity of mitochondrial membrane potential (MMP) and autophagy. LC-ESI/MS was used to identify chemical constituents present in F. officinalis. Chloroform (CF) and ethyl acetate (EF) fractions showed drastic cytotoxic effect on CCRF-CEM and CEM/ADR 5000 cells. NCI-H929 cell line exhibited higher sensitivity against CF, while EF demonstrated its higher cytotoxicity on OPM-2 cells with IC To our knowledge, this is the first report demonstrating the cytotoxicity of F. officinalis extracts in MM cell lines. CF and EF fractions inhibited MM cell proliferation through various modes of actions.
Identifiants
pubmed: 33039632
pii: S0378-8741(20)33344-4
doi: 10.1016/j.jep.2020.113458
pii:
doi:
Substances chimiques
Antineoplastic Agents, Phytogenic
0
Plant Extracts
0
Reactive Oxygen Species
0
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
113458Informations de copyright
Copyright © 2020 Elsevier B.V. All rights reserved.