Galuminox: Preclinical validation of a novel PET tracer for non-invasive imaging of oxidative stress in vivo.
Acute lung injury
Galuminox
Oxidative stress
PET imaging
Journal
Redox biology
ISSN: 2213-2317
Titre abrégé: Redox Biol
Pays: Netherlands
ID NLM: 101605639
Informations de publication
Date de publication:
10 2020
10 2020
Historique:
received:
26
05
2020
revised:
14
08
2020
accepted:
16
08
2020
pubmed:
12
10
2020
medline:
22
6
2021
entrez:
11
10
2020
Statut:
ppublish
Résumé
Overproduction of reactive oxygen species (ROS) is a well-established indicator of ongoing tissue inflammation. However, there is a scarcity of molecular imaging probes capable of providing noninvasive sensitive detection of ROS for allowing longitudinal studies of disease pathology and/or monitoring therapeutic efficacy of ROS scavengers. Herein, we report synthesis and chemical characterization of a novel metalloprobe, Galuminox, a moderately fluorescent agent that detects superoxide and hydrogen peroxide generation. Using live-cell fluorescence imaging analysis, Galuminox demonstrates ability to detect superoxide and monitor effects of ROS-attenuating agents, such as Carvedilol, Dexrazoxane, and mitoTempo in lung epithelial A549 cells. Furthermore, LPS stimulation of A549 cells that either express the mitochondria targeted fluorescent protein Keima or are stained with MitoSOX, a mitochondria-specific superoxide probe, indicates preferential co-localization of Galuminox with mitochondria producing elevated amounts of superoxide. Dynamic PET/CT scans 45 min post tail-vein administration of
Identifiants
pubmed: 33039825
pii: S2213-2317(20)30895-8
doi: 10.1016/j.redox.2020.101690
pmc: PMC7648173
pii:
doi:
Substances chimiques
Reactive Oxygen Species
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
101690Subventions
Organisme : NHLBI NIH HHS
ID : R01 HL111163
Pays : United States
Organisme : NIBIB NIH HHS
ID : P41 EB025815
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL142297
Pays : United States
Organisme : NCI NIH HHS
ID : U24 CA209837
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA091842
Pays : United States
Organisme : NIAID NIH HHS
ID : P01 AI116501
Pays : United States
Organisme : NHLBI NIH HHS
ID : R01 HL094601
Pays : United States
Informations de copyright
Copyright © 2020. Published by Elsevier B.V.
Références
Biochim Biophys Acta. 2010 Jun;1802(6):509-18
pubmed: 20176105
JAMA. 2016 Feb 23;315(8):801-10
pubmed: 26903338
Chem Biol. 2011 Aug 26;18(8):943-8
pubmed: 21867909
Medchemcomm. 2018 Apr 20;9(6):946-950
pubmed: 30108983
Exp Mol Med. 2019 Dec 19;51(12):1-13
pubmed: 31857574
Am J Respir Crit Care Med. 1994 Mar;149(3 Pt 1):818-24
pubmed: 7509706
Am J Physiol Cell Physiol. 2011 Apr;300(4):C723-42
pubmed: 21209361
Nat Rev Immunol. 2003 Feb;3(2):169-76
pubmed: 12563300
Am J Physiol Lung Cell Mol Physiol. 2008 Sep;295(3):L379-99
pubmed: 18621912
Am J Blood Res. 2017 Jul 25;7(4):41-48
pubmed: 28804681
Adv Exp Med Biol. 2017;967:105-137
pubmed: 29047084
Methods Enzymol. 2014;547:225-50
pubmed: 25416361
Redox Biol. 2014 Jan 10;2:206-10
pubmed: 24494194
Biophys J. 2009 Apr 8;96(7):2977-88
pubmed: 19348778
J Forensic Sci. 2016 Sep;61(5):1331-6
pubmed: 27329571
Cell Signal. 2012 May;24(5):981-90
pubmed: 22286106
J Am Chem Soc. 2014 Oct 22;136(42):14742-5
pubmed: 25310369
Chem Soc Rev. 2011 Sep;40(9):4783-804
pubmed: 21629957
Br J Pharmacol. 2007 Jul;151(6):771-8
pubmed: 17519947
Arch Toxicol. 2011 Mar;85(3):209-18
pubmed: 20848084
Reprod Biomed Online. 2004 Oct;9(4):466-8
pubmed: 15511350
Compr Physiol. 2011 Apr;1(2):941-69
pubmed: 23737208
Front Pediatr. 2017 Jul 24;5:162
pubmed: 28791280
PLoS One. 2019 May 23;14(5):e0215579
pubmed: 31120912
J Cell Biol. 2011 Jul 11;194(1):7-15
pubmed: 21746850
Redox Biol. 2019 Sep;26:101273
pubmed: 31325723
React Oxyg Species (Apex). 2016;2(5):361-370
pubmed: 29721549
PLoS One. 2017 Jun 12;12(6):e0179383
pubmed: 28604836
Radiol Clin North Am. 1996 Jan;34(1):33-46
pubmed: 8539352
Org Biomol Chem. 2014 Jul 7;12(25):4421-31
pubmed: 24847866
FASEB J. 2017 Sep;31(9):3729-3745
pubmed: 28592639
Antioxid Redox Signal. 2014 Feb 20;20(6):914-28
pubmed: 23879326
Chemistry. 2017 Jan 5;23(2):254-258
pubmed: 27768812
J Cardiovasc Pharmacol. 1999 Feb;33(2):295-300
pubmed: 10028940
Nature. 2011 Apr 28;472(7344):476-80
pubmed: 21525932
J Immunol. 2014 Jun 15;192(12):5974-83
pubmed: 24795455
JACC Basic Transl Sci. 2018 May 30;3(3):378-390
pubmed: 30062224
Nat Protoc. 2016 Jun;11(6):1057-66
pubmed: 27172166
J Thorac Imaging. 1986 Jul;1(3):25-30
pubmed: 3298678
J Clin Invest. 2017 Sep 1;127(9):3407-3420
pubmed: 28805660
Oxid Med Cell Longev. 2016;2016:3565127
pubmed: 27247702
Ann Transl Med. 2017 Aug;5(16):326
pubmed: 28861423
J Alzheimers Dis. 2019;72(4):981-1017
pubmed: 31744008
J Am Chem Soc. 2011 Jul 20;133(28):10960-5
pubmed: 21631116
Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21316-21
pubmed: 21115844
J Signal Transduct. 2012;2012:646354
pubmed: 21977319
J Biol Chem. 2010 Jan 1;285(1):667-74
pubmed: 19850931
Nucl Med Biol. 2017 Oct;53:14-20
pubmed: 28719807
Ann Transl Med. 2018 Jan;6(2):32
pubmed: 29430449
Crit Care Med. 2019 Nov;47(11):1599-1606
pubmed: 31464770
Plant Methods. 2016 Jan 26;12:5
pubmed: 26819624
J Am Chem Soc. 2010 Apr 28;132(16):5906-15
pubmed: 20361787
J Surg Res. 2015 Apr;194(2):544-550
pubmed: 25479907
Eur Respir J. 1996 May;9(5):1055-62
pubmed: 8793469
Toxicol Appl Pharmacol. 2004 Oct 15;200(2):159-68
pubmed: 15476868
Mol Cells. 2017 Nov 30;40(11):880-887
pubmed: 29145718
Circ Res. 2015 Jan 30;116(3):531-49
pubmed: 25634975
Mutat Res. 2004 Sep;567(1):1-61
pubmed: 15341901
Int J Mol Med. 2018 Jun;41(6):3405-3421
pubmed: 29568857
Nat Protoc. 2017 Aug;12(8):1576-1587
pubmed: 28703790
Sci Rep. 2018 Oct 25;8(1):15764
pubmed: 30361629
Am J Respir Cell Mol Biol. 2009 May;40(5):519-35
pubmed: 18978300
Am J Respir Crit Care Med. 1994 Jun;149(6):1635-9
pubmed: 7516252
Int Arch Allergy Immunol. 2015;166(2):135-49
pubmed: 25824670
Chem Commun (Camb). 2015 May 7;51(37):7931-4
pubmed: 25864583
Circ Res. 2018 Mar 16;122(6):877-902
pubmed: 29700084
Int J Cancer. 2018 Feb 1;142(3):440-448
pubmed: 28940517
Arch Biochem Biophys. 2000 Feb 15;374(2):279-85
pubmed: 10666308
Chest. 1999 Nov;116(5):1347-53
pubmed: 10559098
Biomed Pharmacother. 2017 Dec;96:899-904
pubmed: 29223553
Luminescence. 2004 Sep-Oct;19(5):271-7
pubmed: 15449350
Cell Death Dis. 2018 Jan 25;9(2):119
pubmed: 29371661
Oxid Med Cell Longev. 2019 Dec 14;2019:7536805
pubmed: 31934269
Curr Atheroscler Rep. 2017 Sep 18;19(11):42
pubmed: 28921056
Talanta. 2000 Mar 6;51(3):415-39
pubmed: 18967873