Spatial segmentation and metabolite annotation involved in sperm maturation in the rat epididymis by MALDI imaging mass spectrometry.
MALDI imaging mass spectrometry
epididymis
metabolite annotation
spatial segmentation
three-dimensional imaging mass spectrometry
Journal
Journal of mass spectrometry : JMS
ISSN: 1096-9888
Titre abrégé: J Mass Spectrom
Pays: England
ID NLM: 9504818
Informations de publication
Date de publication:
Dec 2020
Dec 2020
Historique:
received:
10
10
2019
revised:
19
07
2020
accepted:
21
07
2020
entrez:
12
10
2020
pubmed:
13
10
2020
medline:
28
8
2021
Statut:
ppublish
Résumé
Spermatozoa acquire their fertilizing capacity during a complex maturation process that occurs in the epididymis. This process involves a substantial molecular remodeling at the surface of the gamete. Epididymis is divided into three regions (the caput, corpus, and cauda) or into 19 intraregional segments based on histology. Most studies carried out on epididymal maturation have been performed on sperm samples or tissue extracts. Here, we used MALDI imaging mass spectrometry in the positive and negative ion modes combined with spatial segmentation and automated metabolite annotation to study the precise localization of metabolites directly in the rat epididymis. The spatial segmentation revealed that the rat epididymis could be divided into several molecular clusters different from the 19 intraregional segments. The discriminative m/z values that contributed the most to each molecular cluster were then annotated and corresponded mainly to phosphatidylcholines, sphingolipids, glycerophosphates, triacylglycerols, plasmalogens, phosphatidylethanolamines, and lysophosphatidylcholines. A substantial remodeling of lipid composition during epididymal maturation was observed. It was characterized in particular by an increase in the number of sphingolipids and plasmalogens and a decrease in the proportion of triacylglycerols annotated from caput to cauda. Ion images reveal that molecules belonging to the same family can have very different localizations along the epididymis. For some of them, annotation was confirmed by on-tissue MS/MS experiments. A 3D model of the epididymis head was reconstructed from 61 sections analyzed with a lateral resolution of 50 μm and can be used to obtain information on the localization of a given analyte in the whole volume of the tissue.
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
e4633Subventions
Organisme : Biogenouest
Organisme : Conseil Regional de Bretagne
Organisme : Infrastructures en Biologie Sante et Agronomie (IBiSA)
Organisme : and Conseil Régional de Bretagne
Organisme : Biogenouest, Infrastructures en Biologie Santé et Agronomie (IBiSA)
Informations de copyright
© 2020 John Wiley & Sons, Ltd.
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