A whole blood test to measure SARS-CoV-2-specific response in COVID-19 patients.
Adult
Aged
Antigens, Viral
/ immunology
COVID-19
/ blood
COVID-19 Serological Testing
/ methods
Cytokines
/ blood
Female
Humans
Immunoglobulin G
/ blood
Interferon-gamma
/ blood
Male
Middle Aged
SARS-CoV-2
/ immunology
Spike Glycoprotein, Coronavirus
/ immunology
Th1 Cells
/ immunology
Th2 Cells
/ immunology
COVID-19
IFN-γ
Immune response
Multiplex analysis
SARS-CoV-2
Serology response
Specific response
T-cell based tests
Whole blood
Journal
Clinical microbiology and infection : the official publication of the European Society of Clinical Microbiology and Infectious Diseases
ISSN: 1469-0691
Titre abrégé: Clin Microbiol Infect
Pays: England
ID NLM: 9516420
Informations de publication
Date de publication:
Feb 2021
Feb 2021
Historique:
received:
23
07
2020
revised:
26
09
2020
accepted:
26
09
2020
pubmed:
13
10
2020
medline:
26
2
2021
entrez:
12
10
2020
Statut:
ppublish
Résumé
To examine whether specific T-cell-responses to SARS-CoV-2 peptides can be detected in COVID-19 using a whole-blood experimental setting, which may be further explored as a potential diagnostic tool. We evaluated interferon (IFN)-γ levels after stimulating whole-blood with spike and remainder-antigens peptides megapools (MP) derived from SARS-CoV-2 sequences; interleukin (IL)-1β, IL-1RA, IL-2, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-10, IL-12p70, IL-13, IL-15, IL-17A, eotaxin, basic fibroblast growth factor (FGF), granulocyte-colony stimulating factor (G-CSF), granulocyte-macrophage colony-stimulating factor (GM-CSF), IFN-γ, Interferon gamma-induced protein 10 (IP-10), monocyte chemoattractant protein-1 (MCP-1), macrophage inflammatory protein (MIP)-1α, MIP-1β, Platelet-derived growth factor (PDGF), RANTES (regulated on activation, normal T cell expressed and secreted), tumour necrosis factor-alpha (TNF-α), vascular endothelial growth factor (VEGF) were also evaluated. IFN-γ-response to spike and remainder-antigens MPs was significantly increased in 35 COVID-19 patients compared with 29 'no COVID-19' individuals (medians spike-MP: 0.26 vs 0, p = 0.0002; medians remainder-antigens-MP: 0.07 vs 0.02; p = 0.02). This response was detected independently of patients' clinical parameters. IFN-γ-response to SARS-CoV-2-unrelated antigens cytomegalovirus (CMV) and Staphylococcal Enterotoxin B (SEB) was similar in COVID-19 compared with 'no COVID-19' individuals (median CMV: 3.46 vs 5.28, p = 0.16; median SEB: 12.68 vs 15.05; p = 0.1). In response to spike-MPs in COVID-19- compared with 'no COVID-19' -individuals, we found significant higher median of IL-2 (50.08 vs 0, p = 0.0018), IFN-γ (90.16 vs 0, p = 0.01), IL-4 (0.52 vs 0, p = 0.03), IL-13 (0.84 vs 0, p = 0.007) and MCP-1 (4602 vs 359.2, p = 0.05). Immune response to SARS-CoV-2 peptides in a whole-blood assay is associated with COVID-19 and it is characterized by both Th1 and Th2 profile. This experimental approach may be useful for developing new T-cell based diagnostic tests for disease and vaccine settings.
Identifiants
pubmed: 33045370
pii: S1198-743X(20)30605-4
doi: 10.1016/j.cmi.2020.09.051
pmc: PMC7547312
pii:
doi:
Substances chimiques
Antigens, Viral
0
Cytokines
0
IFNG protein, human
0
Immunoglobulin G
0
Spike Glycoprotein, Coronavirus
0
spike protein, SARS-CoV-2
0
Interferon-gamma
82115-62-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
286.e7-286.e13Informations de copyright
Copyright © 2020. Published by Elsevier Ltd.