Differential modulation of short-term plasticity at hippocampal mossy fiber and Schaffer collateral synapses by mitochondrial Ca2.
Animals
Calcium
/ metabolism
Calcium Signaling
/ drug effects
Excitatory Postsynaptic Potentials
/ drug effects
Hippocampus
/ cytology
Male
Mice
Microscopy, Electron
Mitochondria
/ drug effects
Mossy Fibers, Hippocampal
/ drug effects
Neuronal Plasticity
/ drug effects
Onium Compounds
/ pharmacology
Organophosphorus Compounds
/ pharmacology
Patch-Clamp Techniques
Thiazepines
/ pharmacology
Journal
PloS one
ISSN: 1932-6203
Titre abrégé: PLoS One
Pays: United States
ID NLM: 101285081
Informations de publication
Date de publication:
2020
2020
Historique:
received:
12
06
2020
accepted:
29
09
2020
entrez:
13
10
2020
pubmed:
14
10
2020
medline:
15
12
2020
Statut:
epublish
Résumé
Presynaptic mitochondrial Ca2+ plays a critical role in the regulation of synaptic transmission and plasticity. The presynaptic bouton of the hippocampal mossy fiber (MF) is much larger in size than that of the Schaffer collateral (SC) synapse. Here we compare the structural and physiological characteristics of MF and SC presynaptic boutons to reveal functional and mechanistic differences between these two synapses. Our quantitative ultrastructural analysis using electron microscopy show many more mitochondria in MF presynaptic bouton cross-section profiles compared to SC boutons. Consistent with these results, post-tetanic potentiation (PTP), a form of presynaptic short-term plasticity dependent on mitochondrial Ca2+, is reduced by inhibition of mitochondrial Ca2+ release at MF synapses but not at SC synapses. However, blockade of mitochondrial Ca2+ release results in reduction of PTP at SC synapses by disynaptic MF stimulation. Furthermore, inhibition of mitochondrial Ca2+ release selectively decreases frequency facilitation evoked by short trains of presynaptic stimulation at MF synapses, while having no effect at SC synapses. Moreover, depletion of ER Ca2+ stores leads to reduction of PTP at MF synapses, but PTP is unaffected by ER Ca2+ depletion at SC synapses. These findings show that MF and SC synapses differ in presynaptic mitochondrial content as well as mitochondrial Ca2+ dependent synaptic plasticity, highlighting differential regulatory mechanisms of presynaptic plasticity at MF and SC synapses.
Identifiants
pubmed: 33049001
doi: 10.1371/journal.pone.0240610
pii: PONE-D-20-18012
pmc: PMC7553293
doi:
Substances chimiques
7-chloro-5-(2-isopropylphenyl)-3,5-dihydro-4,1-benzothiazepin-2-(1H)-one
0
Onium Compounds
0
Organophosphorus Compounds
0
Thiazepines
0
Calcium
SY7Q814VUP
tetraphenylphosphonium
U9IF5Y5IND
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Langues
eng
Sous-ensembles de citation
IM
Pagination
e0240610Subventions
Organisme : NINDS NIH HHS
ID : R01 NS041783
Pays : United States
Organisme : NIA NIH HHS
ID : RF1 AG063520
Pays : United States
Déclaration de conflit d'intérêts
J.S. is a member of the Board of Directors and has a financial interest in iNeuro Therapeutics, and J.S. also has a financial interest in Apres Therapeutics. Both companies develop therapies for Alzheimer’s disease. J.S.’s interests were reviewed and are managed by BWH and Partners HealthCare in accordance with their conflict of interest policies.
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