Relapse-Associated Transient Synaptic Potentiation Requires Integrin-Mediated Activation of Focal Adhesion Kinase and Cofilin in D1-Expressing Neurons.


Journal

The Journal of neuroscience : the official journal of the Society for Neuroscience
ISSN: 1529-2401
Titre abrégé: J Neurosci
Pays: United States
ID NLM: 8102140

Informations de publication

Date de publication:
28 10 2020
Historique:
received: 11 11 2019
revised: 17 07 2020
accepted: 21 07 2020
pubmed: 15 10 2020
medline: 2 2 2021
entrez: 14 10 2020
Statut: ppublish

Résumé

Relapse to drug use can be initiated by drug-associated cues. The intensity of cue-induced drug seeking in rodent models correlates with the induction of transient synaptic potentiation (t-SP) at glutamatergic synapses in the nucleus accumbens core (NAcore). Matrix metalloproteinases (MMPs) are inducible endopeptidases that degrade extracellular matrix (ECM) proteins, and reveal tripeptide Arginine-Glycine-Aspartate (RGD) domains that bind and signal through integrins. Integrins are heterodimeric receptors composed of αβ subunits, and a primary signaling kinase is focal adhesion kinase (FAK). We previously showed that MMP activation is necessary for and potentiates cued reinstatement of cocaine seeking, and MMP-induced catalysis stimulates β3-integrins to induce t-SP. Here, we determined whether β3-integrin signaling through FAK and cofilin (actin depolymerization factor) is necessary to promote synaptic growth during t-SP. Using a small molecule inhibitor to prevent FAK activation, we blocked cued-induced cocaine reinstatement and increased spine head diameter (d

Identifiants

pubmed: 33051346
pii: JNEUROSCI.2666-19.2020
doi: 10.1523/JNEUROSCI.2666-19.2020
pmc: PMC7605418
doi:

Substances chimiques

Actin Depolymerizing Factors 0
Integrin beta3 0
Receptors, Dopamine D1 0
Focal Adhesion Kinase 1 EC 2.7.10.2
Ptk2 protein, rat EC 2.7.10.2

Types de publication

Journal Article Research Support, N.I.H., Extramural Research Support, N.I.H., Intramural Research Support, U.S. Gov't, Non-P.H.S.

Langues

eng

Sous-ensembles de citation

IM

Pagination

8463-8477

Subventions

Organisme : NIDA NIH HHS
ID : K99 DA047426
Pays : United States
Organisme : NIDA NIH HHS
ID : R00 DA040004
Pays : United States

Informations de copyright

Copyright © 2020 the authors.

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Auteurs

Constanza Garcia-Keller (C)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425 garciake@musc.edu kalivasp@musc.edu.

Michael D Scofield (MD)

Department of Anesthesiology, Medical University of South Carolina, Charleston, South Carolina 29425.

Daniela Neuhofer (D)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Swathi Varanasi (S)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Matthew T Reeves (MT)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Brandon Hughes (B)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Ethan Anderson (E)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Christopher T Richie (CT)

Intramural Research Program, National Institute on Drug Abuse, Baltimore, Maryland 21224.

Carlos Mejias-Aponte (C)

Intramural Research Program, National Institute on Drug Abuse, Baltimore, Maryland 21224.

James Pickel (J)

Intramural Research Program, National Institute of Mental Health, Bethesda, Maryland 20892.

Bruce T Hope (BT)

Intramural Research Program, National Institute on Drug Abuse, Baltimore, Maryland 21224.

Brandon K Harvey (BK)

Intramural Research Program, National Institute on Drug Abuse, Baltimore, Maryland 21224.

Christopher W Cowan (CW)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425.

Peter W Kalivas (PW)

Department of Neuroscience, Medical University of South Carolina, Charleston, South Carolina 29425 garciake@musc.edu kalivasp@musc.edu.

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