A novel microfluidic chip-based sperm-sorting device constructed using design of experiment method.
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
13 10 2020
13 10 2020
Historique:
received:
25
06
2020
accepted:
23
09
2020
entrez:
14
10
2020
pubmed:
15
10
2020
medline:
14
1
2021
Statut:
epublish
Résumé
Microfluidics is proposed as a technique for efficient sperm sorting, to achieve the ultimate goal of resolving infertility problems in livestock industry. Our study aimed to design a microfluidic sperm-sorting device (SSD) through a high-efficacy and cost- and time-effective fabrication process, by using COMSOL Multiphysics simulation and modeling software, and the design of experiment (DOE) method. The eight factors affecting SSD performance were established. The simulation was then run, and statistically significant factors were analyzed. Minitab16 was used to optimize the design modulus factor. By setting the statistical significance at p < 0.05, the factors affecting experimental structure were analyzed. At a desirability of 97.99, the optimal parameters for the microfluidic chip were: angle between sperm and medium inlet chambers (A = 43°), sperm inlet flow rate (B = 0.24 µL min
Identifiants
pubmed: 33051512
doi: 10.1038/s41598-020-73841-3
pii: 10.1038/s41598-020-73841-3
pmc: PMC7553928
doi:
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
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