Label-free imaging of immune cell dynamics in the living retina using adaptive optics.
adaptive optics
immune cell motility
immune response, inflammation
immunology
inflammation
intravital
label-free imaging
mouse
physics of living systems
retina
Journal
eLife
ISSN: 2050-084X
Titre abrégé: Elife
Pays: England
ID NLM: 101579614
Informations de publication
Date de publication:
14 10 2020
14 10 2020
Historique:
received:
07
07
2020
accepted:
15
09
2020
entrez:
14
10
2020
pubmed:
15
10
2020
medline:
20
2
2021
Statut:
epublish
Résumé
Our recent work characterized the movement of single blood cells within the retinal vasculature (Joseph et al. 2019) using adaptive optics ophthalmoscopy. Here, we apply this technique to the context of acute inflammation and discover both infiltrating and tissue-resident immune cells to be visible without any labeling in the living mouse retina using near-infrared light alone. Intravital imaging of immune cells can be negatively impacted by surgical manipulation, exogenous dyes, transgenic manipulation and phototoxicity. These confounds are now overcome, using phase contrast and time-lapse videography to reveal the dynamic behavior of myeloid cells as they interact, extravasate and survey the mouse retina. Cellular motility and differential vascular responses were measured noninvasively and in vivo across hours to months at the same retinal location, from initiation to the resolution of inflammation. As comparable systems are already available for clinical research, this approach could be readily translated to human application.
Identifiants
pubmed: 33052099
doi: 10.7554/eLife.60547
pii: 60547
pmc: PMC7556865
doi:
pii:
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Subventions
Organisme : NEI NIH HHS
ID : R01 EY028293
Pays : United States
Organisme : NIH HHS
ID : P30 EY001319
Pays : United States
Organisme : NIH HHS
ID : R01 EY028293
Pays : United States
Informations de copyright
© 2020, Joseph et al.
Déclaration de conflit d'intérêts
AJ Received funding support from Hoffman-La Roche Inc Hold patents and/or patent applications on adaptive optics technology filed through the University of Rochester. US Patent Application Number: US20190114790A1, "Rapid assessment and visual reporting of local particle velocity". CC No competing interests declared, GF, KD Received funding support from Hoffman-La Roche Inc, JS Received funding support from Hoffman-La Roche Inc Hold patents and/or patent applications on adaptive optics technology filed through the University of Rochester. US Patent #9,844,320 Issued: 12/19/2017, "System and Method for Observing an Object in a Blood Vessel". US Patent Application Number: US20190114790A1, "Rapid assessment and visual reporting of local particle velocity".
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