SARS-CoV-2 RT-PCR profile in 298 Indian COVID-19 patients: a retrospective observational study.
Adolescent
Adult
Aged
Aged, 80 and over
Asymptomatic Diseases
COVID-19
/ diagnosis
COVID-19 Nucleic Acid Testing
/ methods
Child
Child, Preschool
Comorbidity
Diabetes Mellitus
/ diagnosis
Humans
Hypertension
/ diagnosis
India
/ epidemiology
Infant
Infectious Disease Incubation Period
Male
Middle Aged
Nasopharynx
/ virology
Oropharynx
/ virology
RNA, Viral
/ genetics
Retrospective Studies
Reverse Transcriptase Polymerase Chain Reaction
SARS-CoV-2
/ genetics
Severity of Illness Index
COVID-19
RT-PCR
SARS-CoV-2
profile
Journal
Pathogens and disease
ISSN: 2049-632X
Titre abrégé: Pathog Dis
Pays: United States
ID NLM: 101595366
Informations de publication
Date de publication:
09 01 2021
09 01 2021
Historique:
received:
04
06
2020
accepted:
12
10
2020
pubmed:
15
10
2020
medline:
23
1
2021
entrez:
14
10
2020
Statut:
ppublish
Résumé
despite being in the 5th month of pandemic, knowledge with respect to viral dynamics, infectivity and RT-PCR positivity continues to evolve. to analyse the SARS CoV-2 nucleic acid RT-PCR profiles in COVID-19 patients. it was a retrospective, observational study conducted at COVID facilities under AIIMS, New Delhi. patients admitted with laboratory confirmed COVID-19 were eligible for enrolment. Patients with incomplete details, or only single PCR tests were excluded. Data regarding demographic details, comorbidities, treatment received and results of SARS-CoV-2 RT-PCR performed on nasopharyngeal and oropharyngeal swabs, collected at different time points, was retrieved from the hospital records. a total of 298 patients were included, majority were males (75·8%) with mean age of 39·07 years (0·6-88 years). The mean duration from symptom onset to first positive RT-PCR was 4·7 days (SD 3·67), while that of symptom onset to last positive test was 17·83 days (SD 6·22). Proportions of positive RT-PCR tests were 100%, 49%, 24%, 8·7% and 20·6% in the 1st, 2nd, 3rd, 4th and >4 weeks of illness. A total of 12 symptomatic patients had prolonged positive test results even after 3 weeks of symptom onset. Age > = 60 years was associated with prolonged RT-PCR positivity (statistically significant). this study showed that the average period of PCR positivity is more than 2 weeks in COVID-19 patients; elderly patients have prolonged duration of RT-PCR positivity and requires further follow up.
Sections du résumé
BACKGROUND
despite being in the 5th month of pandemic, knowledge with respect to viral dynamics, infectivity and RT-PCR positivity continues to evolve.
AIM
to analyse the SARS CoV-2 nucleic acid RT-PCR profiles in COVID-19 patients.
DESIGN
it was a retrospective, observational study conducted at COVID facilities under AIIMS, New Delhi.
METHODS
patients admitted with laboratory confirmed COVID-19 were eligible for enrolment. Patients with incomplete details, or only single PCR tests were excluded. Data regarding demographic details, comorbidities, treatment received and results of SARS-CoV-2 RT-PCR performed on nasopharyngeal and oropharyngeal swabs, collected at different time points, was retrieved from the hospital records.
RESULTS
a total of 298 patients were included, majority were males (75·8%) with mean age of 39·07 years (0·6-88 years). The mean duration from symptom onset to first positive RT-PCR was 4·7 days (SD 3·67), while that of symptom onset to last positive test was 17·83 days (SD 6·22). Proportions of positive RT-PCR tests were 100%, 49%, 24%, 8·7% and 20·6% in the 1st, 2nd, 3rd, 4th and >4 weeks of illness. A total of 12 symptomatic patients had prolonged positive test results even after 3 weeks of symptom onset. Age > = 60 years was associated with prolonged RT-PCR positivity (statistically significant).
CONCLUSION
this study showed that the average period of PCR positivity is more than 2 weeks in COVID-19 patients; elderly patients have prolonged duration of RT-PCR positivity and requires further follow up.
Identifiants
pubmed: 33053181
pii: 5923555
doi: 10.1093/femspd/ftaa064
pmc: PMC7665504
pii:
doi:
Substances chimiques
RNA, Viral
0
Types de publication
Journal Article
Observational Study
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Informations de copyright
© The Author(s) 2020. Published by Oxford University Press on behalf of FEMS.