BVDV-1 induces interferon-beta gene expression through a pathway involving IRF1, IRF7, and NF-κB activation.
Animals
Bovine Virus Diarrhea-Mucosal Disease
/ genetics
Cattle
Cell Line
Diarrhea Virus 1, Bovine Viral
/ immunology
Epithelial Cells
/ immunology
Gene Expression
/ genetics
Gene Expression Regulation
/ genetics
Interferon Regulatory Factor-1
/ genetics
Interferon Regulatory Factor-7
/ genetics
NF-kappa B
/ genetics
Signal Transduction
/ genetics
IFN-beta
IRF1
IRF7
NF-kB
Journal
Molecular immunology
ISSN: 1872-9142
Titre abrégé: Mol Immunol
Pays: England
ID NLM: 7905289
Informations de publication
Date de publication:
12 2020
12 2020
Historique:
received:
04
11
2019
revised:
07
09
2020
accepted:
27
09
2020
pubmed:
15
10
2020
medline:
26
1
2021
entrez:
14
10
2020
Statut:
ppublish
Résumé
The bovine viral diarrhea virus (BVDV-1) is a pathogen with the capacity to modulate the interferon type I system. To further investigate the effects of BVDV-1 on the production of the immune response, the Madin-Darby bovine kidney cell line was infected with the cytopathic CH001 field isolate of BVDV-1, and the IFNbeta expression profiles were analyzed. The results showed that cpBVDV-1 was able to induce the production of IFNbeta in a way similar to polyinosinic-polycytidylic acid, but with less intensity. Interestingly, all cpBVDV-1 activities were blocked by pharmacological inhibitors of the IRF-1, IRF-7, and NF-κB signaling pathway, and the level of IFNbeta decreased at the level of transcript and protein. These results, together with in silico analyses showing the presence of several regulatory consensus target motifs, suggest that cpBVDV-1 regulates IFNbeta expression in bovines through the activation of several key transcription factors. Collectively, the results suggest that during cpBVDV-1 infection, cross talk is evident between various signaling pathways involved in transcriptional activation of IFNbeta in cattle.
Identifiants
pubmed: 33053462
pii: S0161-5890(20)30494-6
doi: 10.1016/j.molimm.2020.09.018
pii:
doi:
Substances chimiques
Interferon Regulatory Factor-1
0
Interferon Regulatory Factor-7
0
NF-kappa B
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
33-40Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.