Endovascular model of ischemic stroke in swine guided by real-time MRI.
Animals
Brain
/ diagnostic imaging
Contrast Media
/ administration & dosage
Diffusion Tensor Imaging
/ methods
Disease Models, Animal
Gadolinium
/ administration & dosage
Image Processing, Computer-Assisted
/ methods
Ischemic Stroke
/ diagnostic imaging
Male
Neuroimaging
/ methods
Swine
Thrombin
/ administration & dosage
Thrombosis
/ diagnostic imaging
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
14 10 2020
14 10 2020
Historique:
received:
26
05
2020
accepted:
28
09
2020
entrez:
15
10
2020
pubmed:
16
10
2020
medline:
10
4
2021
Statut:
epublish
Résumé
Modeling stroke in animals is essential for testing efficacy of new treatments; however, previous neuroprotective therapies, based on systemic delivery in rodents failed, exposing the need for model with improved clinical relevance. The purpose of this study was to develop endovascular approach for inducing ischemia in swine. To achieve that goal, we used intra-arterial administration of thrombin mixed with gadolinium and visualized the occlusion with real-time MRI. Placement of the microcatheter proximally to rete allowed trans-catheter perfusion of the ipsilateral hemisphere as visualized by contrast-enhanced perfusion MR scans. Dynamic T2*w MRI facilitated visualization of thrombin + Gd solution transiting through cerebral vasculature and persistent hyperintensities indicated occlusion. Area of trans-catheter perfusion dynamically quantified on representative slice before and after thrombin administration (22.20 ± 6.31 cm
Identifiants
pubmed: 33057149
doi: 10.1038/s41598-020-74411-3
pii: 10.1038/s41598-020-74411-3
pmc: PMC7560864
doi:
Substances chimiques
Contrast Media
0
Gadolinium
AU0V1LM3JT
Thrombin
EC 3.4.21.5
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17318Références
Stroke. 2009 Jun;40(6):2244-50
pubmed: 19246690
Stroke. 2013 Mar;44(3):720-6
pubmed: 23362081
Exp Transl Stroke Med. 2014 Mar 23;6(1):5
pubmed: 24655785
Ann Neurol. 2005 Jun;57(6):874-82
pubmed: 15929052
Metab Brain Dis. 2015 Apr;30(2):461-7
pubmed: 24526567
Can Assoc Radiol J. 2004 Dec;55(5):326-9
pubmed: 15646463
N Engl J Med. 2013 Mar 7;368(10):914-23
pubmed: 23394476
AJNR Am J Neuroradiol. 2004 Feb;25(2):329-32
pubmed: 14970041
Stroke. 2000 Dec;31(12):3054-63
pubmed: 11108772
J Neurosurg. 2006 Feb;104(2 Suppl):123-32
pubmed: 16506500
Brain Res. 2010 Jun 2;1334:84-92
pubmed: 20382136
Pract Neurol. 2017 Aug;17(4):252-265
pubmed: 28647705
Lab Invest. 2013 Jul;93(7):760-7
pubmed: 23648562
PLoS One. 2015 Nov 06;10(11):e0142251
pubmed: 26545253
Brain Sci. 2019 Jan 21;9(1):
pubmed: 30669616
Stroke. 2018 May;49(5):1075-1082
pubmed: 29669876
Restor Neurol Neurosci. 2015;33(2):131-42
pubmed: 25588459
Ann Neurol. 2006 Mar;59(3):467-77
pubmed: 16453316
J Cereb Blood Flow Metab. 2017 Jul;37(7):2346-2358
pubmed: 27618834
J Neurosci Res. 2009 Dec;87(16):3554-67
pubmed: 19642203
Stroke. 2011 Mar;42(3):825-9
pubmed: 21273569
Stroke. 2017 Nov;48(11):3165-3167
pubmed: 28931616
Ann Neurol. 2019 Jul;86(1):5-16
pubmed: 31020699
J Neurointerv Surg. 2016 Apr;8(4):418-22
pubmed: 25676148
Brain Res. 2008 Oct 21;1236:30-8
pubmed: 18722359
J Neurosurg. 2018 Nov 23;:1-11
pubmed: 30497166
J Cereb Blood Flow Metab. 2008 Dec;28(12):1951-64
pubmed: 18698332
J Neurosci Methods. 2001 Jan 30;105(1):45-53
pubmed: 11166365
N Engl J Med. 1995 Dec 14;333(24):1581-7
pubmed: 7477192
Cell Mol Neurobiol. 2015 Apr;35(3):399-406
pubmed: 25398358
Stem Cells Dev. 2009 Dec;18(10):1501-14
pubmed: 19301956
Stem Cells. 2017 May;35(5):1141-1153
pubmed: 28207204
Circulation. 2018 Mar 20;137(12):e67-e492
pubmed: 29386200
Stroke. 2014 Dec;45(12):3618-24
pubmed: 25378424
AJNR Am J Neuroradiol. 2006 Jun-Jul;27(6):1357-61
pubmed: 16775297
Neurosci Lett. 2016 Dec 2;635:1-7
pubmed: 27773792
Brain Res Bull. 2016 Oct;127:134-140
pubmed: 27496066
N Engl J Med. 2013 Mar 7;368(10):893-903
pubmed: 23390923
J Stroke Cerebrovasc Dis. 2016 Sep;25(9):2177-83
pubmed: 27468660
Brain Res Bull. 1999 May;49(1-2):1-137
pubmed: 10466025
Lancet. 2016 Apr 23;387(10029):1723-31
pubmed: 26898852
Stroke. 2008 May;39(5):1613-5
pubmed: 18340097
Behav Brain Res. 2013 Aug 1;250:222-9
pubmed: 23685323
J Cereb Blood Flow Metab. 2019 Mar;39(3):375-394
pubmed: 30732549