Ethidium and propidium monoazide: comparison of potential toxicity on Vibrio sp. viability.
Anti-Bacterial Agents
/ pharmacology
Azides
/ pharmacology
Ecosystem
Gastroenteritis
/ microbiology
Humans
Microbial Sensitivity Tests
Microbial Viability
/ drug effects
Propidium
/ analogs & derivatives
Real-Time Polymerase Chain Reaction
/ methods
Vibrio cholerae
/ drug effects
Vibrio vulnificus
/ drug effects
Water Microbiology
Vibrio
EMA
PMA
VBNC
toxicity
Journal
Letters in applied microbiology
ISSN: 1472-765X
Titre abrégé: Lett Appl Microbiol
Pays: England
ID NLM: 8510094
Informations de publication
Date de publication:
Mar 2021
Mar 2021
Historique:
received:
19
06
2020
revised:
07
10
2020
accepted:
07
10
2020
pubmed:
16
10
2020
medline:
14
4
2021
entrez:
15
10
2020
Statut:
ppublish
Résumé
Vibrio sp., ubiquitous in the aquatic ecosystem, are bacteria of interest because of their involvement in human health, causing gastroenteritis after ingestion of seafood, as well as their role in vibriosis leading to severe losses in aquaculture production. Their ability to enter a viable but non-culturable (VBNC) state under stressful environmental conditions may lead to underestimation of the Vibrio population by traditional microbiological enumeration methods. As a result, using molecular methods in combination with EMA or PMA allows the detection of viable (VBNC and culturable viable) cells. In this study, the impact of the EMA and PMA was tested at different concentrations on the viability of several Vibrio species. We compared the toxicity of these two DNA-binding dyes to determine the best pretreatment to use with qPCR to discriminate between viable and dead Vibrio cells. Our results showed that EMA displayed lethal effects for each strain of V. cholerae and V. vulnificus tested. In contrast, the concentrations of PMA tested had no toxic effect on the viability of Vibrio cells studied. These results may help to achieve optimal PMA-qPCR methods to detect viable Vibrio sp. cells in food and environmental samples.
Substances chimiques
Anti-Bacterial Agents
0
Azides
0
propidium monoazide
0
Propidium
36015-30-2
8-azidoethidium
69498-50-6
Types de publication
Journal Article
Langues
eng
Sous-ensembles de citation
IM
Pagination
245-250Informations de copyright
© 2020 The Society for Applied Microbiology.
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