Functional analysis of Pdp1 and vrille in the circadian system of a cricket.
Circadian clock
Insect
Molecular oscillatory mechanism
Pdp1
vrille
Journal
Journal of insect physiology
ISSN: 1879-1611
Titre abrégé: J Insect Physiol
Pays: England
ID NLM: 2985080R
Informations de publication
Date de publication:
Historique:
received:
28
12
2019
revised:
04
09
2020
accepted:
09
10
2020
pubmed:
16
10
2020
medline:
27
1
2021
entrez:
15
10
2020
Statut:
ppublish
Résumé
Circadian rhythms are generated by a circadian clock for which oscillations are based on the rhythmic expression of the so-called clock genes. The present study investigated the role of Gryllus bimaculatus vrille (Gb'vri) and Par domain protein 1 (Gb'Pdp1) in the circadian clock of the cricket Gryllus bimaculatus. Structural analysis of Gb'vri and Gb'Pdp1 cDNAs revealed that they are a member of the bZIP transcription factors. Under light/dark cycles (LD) both genes were rhythmically expressed in the clock tissue, the optic lobes, whereas the rhythm diminished under constant darkness (DD). Gb'vri and Gb'Pdp1 mRNA levels were significantly reduced by RNA interference (RNAi) of Gb'Clk and Gb'cyc, suggesting they are controlled by Gb'CLK/Gb'CYC. RNAi of Gb'vri and Gb'Pdp1 had little effect on locomotor rhythms, although their effects became visible when treated together with Gb'cyc
Identifiants
pubmed: 33058831
pii: S0022-1910(20)30300-0
doi: 10.1016/j.jinsphys.2020.104156
pii:
doi:
Substances chimiques
Basic-Leucine Zipper Transcription Factors
0
Insect Proteins
0
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
104156Informations de copyright
Copyright © 2020 Elsevier Ltd. All rights reserved.