Elastin calcification in in vitro models and its prevention by MGP's N-terminal peptide.
Elastin
Elastocalcinosis
Matrix Gla protein
Medial calcification
Journal
Journal of structural biology
ISSN: 1095-8657
Titre abrégé: J Struct Biol
Pays: United States
ID NLM: 9011206
Informations de publication
Date de publication:
03 2021
03 2021
Historique:
received:
01
06
2020
revised:
01
10
2020
accepted:
03
10
2020
pubmed:
16
10
2020
medline:
15
12
2021
entrez:
15
10
2020
Statut:
ppublish
Résumé
Medial calcification has been associated with diabetes, chronic kidney disease, and genetic disorders like pseudoxanthoma elasticum. Recently, we showed that genetic reduction of arterial elastin content reduces the severity of medial calcification in matrix Gla protein (MGP)-deficient and Eln haploinsufficient Mgp-/-;Eln+/- mice. This study suggests that there might be a direct effect of elastin amount on medial calcification. We studied this using novel in vitro systems, which are based on elastin or elastin-like polypeptides. We first examined the mineral deposition properties of a transfected pigmented epithelial cell line that expresses elastin and other elastic lamina proteins. When grown in inorganic phosphate-supplemented medium, these cells deposited calcium phosphate minerals, which could be prevented by an N'-terminal peptide of MGP (m3pS) carrying phosphorylated serine residues. We next confirmed these findings using a cell-free elastin-like polypeptide (ELP
Identifiants
pubmed: 33059036
pii: S1047-8477(20)30210-0
doi: 10.1016/j.jsb.2020.107637
pii:
doi:
Substances chimiques
Calcium-Binding Proteins
0
Extracellular Matrix Proteins
0
Minerals
0
Peptides
0
Elastin
9007-58-3
Types de publication
Journal Article
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
107637Subventions
Organisme : CIHR
ID : PJT-168888
Pays : Canada
Informations de copyright
Copyright © 2020. Published by Elsevier Inc.