Unusual zwitterionic catalytic site of SARS-CoV-2 main protease revealed by neutron crystallography.

Catalytic Domain Coronavirus 3C Proteases / chemistry Crystallography, X-Ray Models, Molecular Neutrons SARS-CoV-2 / genetics
3CL Mpro 3CL main protease SARS–CoV-2 SARS–CoV-2 3CL main protease X-ray crystallography drug design enzyme mechanism hydrogen bond joint neutron neutron diffraction protonation state room temperature viral protease

Journal

The Journal of biological chemistry
ISSN: 1083-351X
Titre abrégé: J Biol Chem
Pays: United States
ID NLM: 2985121R

Informations de publication

Date de publication:
11 12 2020
Historique:
received: 22 09 2020
revised: 12 10 2020
pubmed: 17 10 2020
medline: 22 12 2020
entrez: 16 10 2020
Statut: ppublish

Résumé

The main protease (3CL M

Identifiants

DOI: 10.1074/jbc.AC120.016154 PMID: 33060199 PMC: PMC7832724
pubmed: 33060199
pii: S0021-9258(17)50622-5
doi: 10.1074/jbc.AC120.016154
pmc: PMC7832724
pii:
doi:

Substances chimiques

3C-like protease, SARS coronavirus EC 3.4.22.-
Coronavirus 3C Proteases EC 3.4.22.28

Types de publication

Journal Article Research Support, Non-U.S. Gov't

Langues

eng

Sous-ensembles de citation

IM

Pagination

17365-17373

Informations de copyright

© 2020 Kneller et al.

Déclaration de conflit d'intérêts

Conflict of interest—The authors declare that they have no conflicts of interest with the contents of this article.

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Auteurs

Daniel W Kneller (DW)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Gwyndalyn Phillips (G)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Kevin L Weiss (KL)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Swati Pant (S)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Qiu Zhang (Q)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Hugh M O'Neill (HM)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA.

Leighton Coates (L)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA; Second Target Station, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA. Electronic address: coatesl@ornl.gov.

Andrey Kovalevsky (A)

Neutron Scattering Division, Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA; National Virtual Biotechnology Laboratory, United States Department of Energy, Washington, DC, USA. Electronic address: kovalevskyay@ornl.gov.

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Classifications MeSH

questionsmedicales.fr › Phénomènes chimiques › Phénomènes biochimiques › Conformation moléculaire › Conformation des protéines › Éléments structuraux des protéines › Domaine catalytique › Unusual zwitterionic catalytic site of...
questionsmedicales.fr › Acides aminés, peptides et protéines › Protéines › Protéines virales › Protéines virales non structurales › Protéases virales › Protéases virales 3C › Protéases 3C des coronavirus › Unusual zwitterionic catalytic site of...
questionsmedicales.fr › Techniques d'investigation › Techniques de chimie analytique › Cristallographie aux rayons X › Unusual zwitterionic catalytic site of...
questionsmedicales.fr › Techniques d'investigation › Modèles théoriques › Modèles moléculaires › Unusual zwitterionic catalytic site of...
questionsmedicales.fr › Phénomènes physiques › Particules élémentaires › Nucléons › Neutrons › Unusual zwitterionic catalytic site of...
questionsmedicales.fr › Virus › Virus à ARN › Virus à ARN à brin positif › Nidovirales › Coronaviridae › Coronavirus › Betacoronavirus › Virus du SRAS › SARS-CoV-2 › Unusual zwitterionic catalytic site of...