A site-specific map of the human plasma glycome and its age and gender-associated alterations.
Adult
Age Factors
Aged
Aged, 80 and over
Biomarkers
/ blood
Blood Proteins
Female
Glycomics
Glycopeptides
/ blood
Glycosylation
Healthy Volunteers
Humans
Immunoglobulin G
/ blood
Likelihood Functions
Male
Middle Aged
Polysaccharides
/ blood
Sex Factors
Spectrometry, Mass, Electrospray Ionization
Young Adult
Journal
Scientific reports
ISSN: 2045-2322
Titre abrégé: Sci Rep
Pays: England
ID NLM: 101563288
Informations de publication
Date de publication:
15 10 2020
15 10 2020
Historique:
received:
14
04
2020
accepted:
10
09
2020
entrez:
16
10
2020
pubmed:
17
10
2020
medline:
10
3
2021
Statut:
epublish
Résumé
Alterations in the human glycome have been associated with cancer and autoimmunity. Thus, constructing a site-specific map of the human glycome for biomarker research and discovery has been a highly sought-after objective. However, due to analytical barriers, comprehensive site-specific glycoprofiling is difficult to perform. To develop a platform to detect easily quantifiable, site-specific, disease-associated glycan alterations for clinical applications, we have adapted the multiple reaction monitoring mass spectrometry method for use in glycan biomarker research. The adaptations allow for highly precise site-specific glycan monitoring with minimum sample prep. Using this technique, we successfully mapped out the relative abundances of the most common 159 glycopeptides in the plasma of 97 healthy volunteers. This plasma glycome map revealed 796 significant (FDR < 0.05) site-specific inter-protein and intra-protein glycan associations, of which the vast majority were previously unknown. Since age and gender are relevant covariants in biomarker research, these variables were also characterized. 13 glycopeptides were found to be associated with gender and 41 to be associated with age. Using just five age-associated glycopeptides, a highly accurate age prediction model was constructed and validated (r
Identifiants
pubmed: 33060657
doi: 10.1038/s41598-020-73588-x
pii: 10.1038/s41598-020-73588-x
pmc: PMC7567094
doi:
Substances chimiques
Biomarkers
0
Blood Proteins
0
Glycopeptides
0
Immunoglobulin G
0
Polysaccharides
0
Types de publication
Journal Article
Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't
Langues
eng
Sous-ensembles de citation
IM
Pagination
17505Subventions
Organisme : Howard Hughes Medical Institute
Pays : United States
Organisme : NCI NIH HHS
ID : P30 CA093373
Pays : United States
Organisme : NIH HHS
ID : DP2 OD008752
Pays : United States
Références
J Proteomics. 2012 Feb 2;75(4):1318-29
pubmed: 22120122
Clin Exp Nephrol. 2006 Sep;10(3):180-5
pubmed: 17009075
Glycoconj J. 2012 Jan;29(1):57-66
pubmed: 22179780
J Mass Spectrom. 2011 Mar;46(3):298-312
pubmed: 21394846
J Proteome Res. 2015 Dec 4;14(12):5179-92
pubmed: 26510530
Nature. 1985 Aug 1-7;316(6027):452-7
pubmed: 3927174
J Autoimmun. 1989 Apr;2(2):101-14
pubmed: 2504180
AIDS. 2005 Mar 4;19(4):381-9
pubmed: 15750391
Lancet. 1988 Apr 30;1(8592):966-9
pubmed: 2896829
Mol Cell Proteomics. 2012 Apr;11(4):M111.014563
pubmed: 22184099
Nat Immunol. 2001 Sep;2(9):777-80
pubmed: 11526384
Age Ageing. 2011 Sep;40(5):568-75
pubmed: 21807702
J Chromatogr B Analyt Technol Biomed Life Sci. 2009 May 1;877(13):1229-39
pubmed: 19041286
CA Cancer J Clin. 2017 Jan;67(1):7-30
pubmed: 28055103
Trends Analyt Chem. 2012 Feb 1;32:1-14
pubmed: 22345829
Biochim Biophys Acta. 1999 Dec 6;1473(1):4-8
pubmed: 10580125
J Autoimmun. 1997 Feb;10(1):77-85
pubmed: 9080302
J Exp Med. 1988 May 1;167(5):1731-6
pubmed: 3367097
Rapid Commun Mass Spectrom. 2005;19(14):1943-50
pubmed: 15954168
J Proteome Res. 2011 Apr 1;10(4):1667-74
pubmed: 21184610
Anal Chem. 2019 Apr 16;91(8):5433-5445
pubmed: 30882205
Proteomics Clin Appl. 2013 Oct;7(9-10):664-76
pubmed: 23640812
Anal Chem. 2013 Sep 17;85(18):8585-93
pubmed: 23944609
Mol Cell Proteomics. 2014 Jun;13(6):1598-610
pubmed: 24719452
J Proteomics. 2012 Jun 6;75(10):2824-34
pubmed: 22365975
J Proteome Res. 2011 Jan 7;10(1):143-52
pubmed: 20672848
J Proteome Res. 2018 Jan 5;17(1):222-233
pubmed: 29207246
Int J Legal Med. 2015 Sep;129(5):955-61
pubmed: 25787342
Glycobiology. 2007 Dec;17(12):1344-56
pubmed: 17884841
J Autoimmun. 2015 Feb;57:1-13
pubmed: 25578468
Mol Biosyst. 2009 Oct;5(10):1087-104
pubmed: 19756298
Chromatographia. 2015 Mar 1;78(5-6):335-342
pubmed: 25892741
Ann N Y Acad Sci. 2006 Nov;1089:487-95
pubmed: 17261791
Biochim Biophys Acta. 2006 Apr;1760(4):669-77
pubmed: 16413679
Electrophoresis. 2013 Aug;34(16):2407-16
pubmed: 23766031