Suppression of Inflammatory Cytokines Expression with Bitter Melon (
CYP27B1
Momordica charantia
alternative and complementary therapeutics
anti-inflammatory
immunohistochemistry
inflammatory bowel disease
inflammatory cytokines
trinitrobenzenesulfonic acid
ulcerative colitis
vitamin D
Journal
Journal of translational internal medicine
ISSN: 2450-131X
Titre abrégé: J Transl Int Med
Pays: Poland
ID NLM: 101673826
Informations de publication
Date de publication:
Sep 2020
Sep 2020
Historique:
entrez:
16
10
2020
pubmed:
17
10
2020
medline:
17
10
2020
Statut:
epublish
Résumé
This study was aimed to elucidate the molecular mechanism of After the induction of the experimental colitis, the animals were treated with MCh (4 g/kg/day) for 14 consecutive days by intragastric gavage. The colonic tissue expression levels of C-C motif chemokine ligand 17 (CCL-17), interleukin (IL)-1β, IL-6, IL-23, interferon-γ (IFN-γ), nuclear factor kappa B (NF-kB), and tumor necrosis factor-α (TNF-α), were determined at both mRNA and protein levels to estimate the effect of The body weights from TNBS-instigated colitis rats were found to be significantly lower than untreated animals. Also, the IFN-γ, IL-1β, IL-6, Il-23, TNF-α, CCL-17, and NF-kB mRNA and protein levels were increased significantly from 1.86-4.91-fold and 1.46-5.50-fold, respectively, in the TNBS-instigated colitis group as compared to the control. Both the MCh and prednisolone treatment significantly reduced the bodyweight loss. It also restored the induced colonic tissue levels of IL-1β, IL-6, IFN-γ, and TNF-α to normal levels seen in untreated animals. These results were also supported with the histochemical staining of the colonic tissues from both control and treated animals. The presented data strongly suggests that
Sections du résumé
BACKGROUND AND OBJECTIVE
OBJECTIVE
This study was aimed to elucidate the molecular mechanism of
METHODS
METHODS
After the induction of the experimental colitis, the animals were treated with MCh (4 g/kg/day) for 14 consecutive days by intragastric gavage. The colonic tissue expression levels of C-C motif chemokine ligand 17 (CCL-17), interleukin (IL)-1β, IL-6, IL-23, interferon-γ (IFN-γ), nuclear factor kappa B (NF-kB), and tumor necrosis factor-α (TNF-α), were determined at both mRNA and protein levels to estimate the effect of
RESULTS
RESULTS
The body weights from TNBS-instigated colitis rats were found to be significantly lower than untreated animals. Also, the IFN-γ, IL-1β, IL-6, Il-23, TNF-α, CCL-17, and NF-kB mRNA and protein levels were increased significantly from 1.86-4.91-fold and 1.46-5.50-fold, respectively, in the TNBS-instigated colitis group as compared to the control. Both the MCh and prednisolone treatment significantly reduced the bodyweight loss. It also restored the induced colonic tissue levels of IL-1β, IL-6, IFN-γ, and TNF-α to normal levels seen in untreated animals. These results were also supported with the histochemical staining of the colonic tissues from both control and treated animals.
CONCLUSION
CONCLUSIONS
The presented data strongly suggests that
Identifiants
pubmed: 33062594
doi: 10.2478/jtim-2020-0027
pii: jtim-2020-0027
pmc: PMC7534491
doi:
Types de publication
Journal Article
Langues
eng
Pagination
177-187Informations de copyright
© 2020 Asli Semiz, Ozden Ozgun Acar, Hulya Cetin, Gurkan Semiz, Alaattin Sen, published by Sciendo.
Déclaration de conflit d'intérêts
Conflict of Interest The authors declared that they have no conflict of interest.
Références
Pathol Int. 2012 Jun;62(6):368-80
pubmed: 22612505
J Exp Med. 2005 Jan 17;201(2):233-40
pubmed: 15657292
J Pathol. 2008 Jan;214(2):231-41
pubmed: 18161758
Eur J Pharmacol. 2007 Feb 28;557(2-3):221-9
pubmed: 17184768
World J Gastroenterol. 2019 Jun 21;25(23):2846-2862
pubmed: 31249444
Front Pharmacol. 2014 Jan 10;4:177
pubmed: 24454289
Trends Mol Med. 2009 May;15(5):199-207
pubmed: 19362058
Int Immunopharmacol. 2017 Sep;50:152-160
pubmed: 28666238
Eur J Pharmacol. 2011 Nov 16;670(1):229-35
pubmed: 21925164
Cytokine. 2014 Dec;70(2):81-6
pubmed: 25132256
World J Gastroenterol. 2012 Nov 7;18(41):5848-61
pubmed: 23139600
Turk J Biol. 2018 Dec 10;42(6):506-516
pubmed: 30983870
Mediators Inflamm. 2014;2014:312484
pubmed: 25276054
Biochem Biophys Res Commun. 2005 Aug 19;334(1):254-62
pubmed: 15993846
J Exp Med. 2006 Oct 30;203(11):2485-94
pubmed: 17030948
Gastroenterology. 2012 Feb;142(2):335-45
pubmed: 22057112
Eur J Immunol. 1997 Jul;27(7):1743-50
pubmed: 9247586
Nature. 1970 Aug 15;227(5259):680-5
pubmed: 5432063
J Clin Invest. 2006 May;116(5):1218-22
pubmed: 16670765
Int Immunopharmacol. 2015 Mar;25(1):55-64
pubmed: 25614226
Oncotarget. 2017 Dec 14;9(6):7204-7218
pubmed: 29467962
Nat Clin Pract Gastroenterol Hepatol. 2006 Jul;3(7):390-407
pubmed: 16819502
J Immunol. 2000 May 1;164(9):4878-82
pubmed: 10779797
J Clin Invest. 2006 May;116(5):1310-6
pubmed: 16670770
Oncogene. 2006 Oct 30;25(51):6680-4
pubmed: 17072321
Cold Spring Harb Perspect Biol. 2015 Jan 29;7(5):
pubmed: 25635046
Gastroenterol Res Pract. 2012;2012:362536
pubmed: 22888336
J Ethnopharmacol. 2019 Mar 1;231:39-49
pubmed: 30170079
Mediators Inflamm. 2006;2006(5):92642
pubmed: 17392580
Cold Spring Harb Perspect Biol. 2014 Sep 04;6(10):a016295
pubmed: 25190079
Eur J Pharmacol. 2016 Dec 15;793:28-34
pubmed: 27983945
Arch Oral Biol. 2015 Dec;60(12):1708-13
pubmed: 26431827
Immunity. 2000 Nov;13(5):715-25
pubmed: 11114383
Semin Immunol. 2014 Feb;26(1):80-7
pubmed: 24572992
Inflamm Bowel Dis. 2006 Jul;12(7):612-8
pubmed: 16804399
Arch Dermatol Res. 2011 Jul;303(5):351-60
pubmed: 21190116
Comp Biochem Physiol C Toxicol Pharmacol. 2000 Jul;126(3):235-44
pubmed: 11048673
J Immunol. 2017 Jul 1;199(1):9-16
pubmed: 28630108
Gut. 2001 Jul;49(1):35-41
pubmed: 11413108
J Exp Med. 1996 Jun 1;183(6):2605-16
pubmed: 8676081
J Neuroimmunol. 2016 Sep 15;298:106-16
pubmed: 27609283
Proc Soc Exp Biol Med. 1955 Oct;90(1):210-3
pubmed: 13273400
Kidney Int. 2010 Jul;78(2):140-5
pubmed: 20182414
J Biochem Mol Toxicol. 1998;12(2):103-13
pubmed: 9443067
J Exp Med. 1998 Apr 20;187(8):1225-34
pubmed: 9547334
Aliment Pharmacol Ther. 1996;10 Suppl 2:36-42; discussion 43-4
pubmed: 8899099
J Ethnopharmacol. 2000 Jul;71(1-2):77-82
pubmed: 10904148
N Engl J Med. 2009 Nov 19;361(21):2066-78
pubmed: 19923578
Cancer Res. 2007 Nov 15;67(22):10766-73
pubmed: 18006820
Gut. 1994 May;35(5):696-700
pubmed: 8200569
Eur J Pharmacol. 2016 Nov 15;791:8-24
pubmed: 27568833
World J Gastroenterol. 2009 Feb 21;15(7):788-803
pubmed: 19230040
Nat Rev Immunol. 2003 Sep;3(9):745-56
pubmed: 12949498
J Ethnopharmacol. 1999 Sep;66(3):289-93
pubmed: 10473175
Ann Med Surg (Lond). 2016 Aug 19;11:9-15
pubmed: 27656280
J Nat Prod. 2006 Jul;69(7):1018-21
pubmed: 16872136
Gastroenterology. 1999 Oct;117(4):866-76
pubmed: 10500069
Int J Biol Macromol. 2018 May;111:193-199
pubmed: 29307809
Biomed Res Int. 2013;2013:358945
pubmed: 23971029
Biomed Pharmacother. 2017 Jul;91:1113-1121
pubmed: 28531922
Turk J Gastroenterol. 2005 Jun;16(2):85-8
pubmed: 16252198
Trends Biochem Sci. 2004 Dec;29(12):664-73
pubmed: 15544953
Digestion. 1998;59(1):73-8
pubmed: 9468102
Mol Med Rep. 2013 Aug;8(2):446-50
pubmed: 23778962
Chem Immunol Allergy. 2007;93:181-194
pubmed: 17369706
Nucleic Acids Res. 2019 Jan 8;47(D1):D607-D613
pubmed: 30476243
Gastroenterology. 1989 Aug;97(2):326-37
pubmed: 2545504
Molecules. 2016 Oct 01;21(10):
pubmed: 27706084
Phytother Res. 2009 Dec;23(12):1763-70
pubmed: 19441062
Arch Pharm (Weinheim). 2017 Nov;350(11):
pubmed: 28960496
Gut. 1990 Jun;31(6):686-9
pubmed: 2379873
Anal Biochem. 1985 Oct;150(1):76-85
pubmed: 3843705
Endocrinology. 2017 Nov 1;158(11):4064-4075
pubmed: 28938443
Gastroenterology. 1993 Mar;104(3):749-58
pubmed: 8440434
Surg Clin North Am. 2019 Dec;99(6):1051-1062
pubmed: 31676047
J Ethnopharmacol. 2000 Jul;71(1-2):23-43
pubmed: 10904144
Surg Clin North Am. 2019 Dec;99(6):1197-1207
pubmed: 31676058
J Investig Med. 2011 Aug;59(6):881-6
pubmed: 21527855
Phytother Res. 2010 Jul;24(7):949-63
pubmed: 20577970
Molecules. 2014 Aug 21;19(8):12777-88
pubmed: 25153878
World J Gastroenterol. 2013 May 7;19(17):2638-49
pubmed: 23674871
Gastroenterology. 2011 May;140(6):1756-1767
pubmed: 21530742
Gastroenterology. 1993 May;104(5):1285-92
pubmed: 7683293
Signal Transduct Target Ther. 2017;2:
pubmed: 29158945
Am J Respir Cell Mol Biol. 1997 Jul;17(1):3-9
pubmed: 9224203
CMAJ. 2005 Feb 1;172(3):367-79
pubmed: 15684121
J Ethnopharmacol. 2005 Jan 4;96(1-2):49-55
pubmed: 15588650
Am J Clin Pathol. 1957 Jul;28(1):56-63
pubmed: 13458125